Anti-Muscarinic Acetylcholine Receptor 2 antibody [31-1D1]
| Name | Anti-Muscarinic Acetylcholine Receptor 2 antibody [31-1D1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab2805 |
| Prices | $391.00 |
| Sizes | 125 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 31-1D1 |
| Applications | WB FC ICC/IF ICC/IF ELISA IHC-P IP |
| Species Reactivities | Mouse, Rat, Human, Pig |
| Antigen | Full length native protein (purified) corresponding to Pig Muscarinic Acetylcholine Receptor 2 |
| Description | Mouse Monoclonal |
| Gene | CHRM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing Muscarinic Acetylcholine Receptor 2 (ab2805) or without primary antibody (negative control) overnight at 4�C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
ICC/IF image of ab2805 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2805, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![All lanes : Anti-Muscarinic Acetylcholine Receptor 2 antibody [31-1D1] (ab2805) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 3 : Brain (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/26154_Muscarinic-Acetylcholine-Receptor-2-Primary-antibodies-ab2805-3.jpg)
All lanes : Anti-Muscarinic Acetylcholine Receptor 2 antibody [31-1D1] (ab2805) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 3 : Brain (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Muscarinic acetylcholine receptor modulation of mu (mu) opioid receptors in adult - Muscarinic acetylcholine receptor modulation of mu (mu) opioid receptors in adult
Margas W, Mahmoud S, Ruiz-Velasco V. J Neurophysiol. 2010 Jan;103(1):172-82.
Functional nicotinic and muscarinic receptors on mesenchymal stem cells. - Functional nicotinic and muscarinic receptors on mesenchymal stem cells.
Hoogduijn MJ, Cheng A, Genever PG. Stem Cells Dev. 2009 Jan-Feb;18(1):103-12.
Isolation and characterization of monoclonal antibodies specific for the cardiac - Isolation and characterization of monoclonal antibodies specific for the cardiac
Luetje CW, Brumwell C, Norman MG, Peterson GL, Schimerlik MI, Nathanson NM. Biochemistry. 1987 Nov 3;26(22):6892-6.