Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling MYBBP1A with ab99361 at 1/1000 (1µg/ml). Detection: DAB.
ICC/IF image of ab99361 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99361 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%, 5min) HepG2, HEk293, and MCF-7 cells at 1ug/ml.
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99361 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane Western Blot: rabbit anti-MYBBP1A antibody at 1 µg/ml developed using the ECL technique Exposure time : 1 second