Anti-MyD88 antibody
| Name | Anti-MyD88 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab135693 |
| Prices | $385.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 143-172 of Human MyD88 |
| Description | Rabbit Polyclonal |
| Gene | MYD88 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab135693 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135693 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-MyD88 antibody (ab135693) at 1/100 dilution + HepG2 cell line lysates at 35 µg
Anti-MyD88 antibody (ab135693) at 2 µg/ml + Mouse lung tissue lysates at 35 µg
Immunohistochemical analysis of formalin fixed, paraffin embedded Human lung carcinoma tissue labelling MyD88 with ab135693 at 1/50 dilution followed by peroxidase conjugated secondary antibody and DAB staining.
Flow Cytometric analysis of NCI-H460 cells labelling MyD88 with ab135693 at 1/10 dilution (green) compared to a negative control cell (blue). ITC conjugated Goat anti Rabbit secondary antibodies were used for the analysis.
Product References
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Huang JM, Zhang GN, Shi Y, Zha X, Zhu Y, Wang MM, Lin Q, Wang W, Lu HY, Ma SQ, Cheng J, Deng BF. Sci Rep. 2014 Jan 23;4:3840.
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Liang B, Chen R, Wang T, Cao L, Liu Y, Yin F, Zhu M, Fan X, Liang Y, Zhang L, Guo Y, Zhao J. Clin Cancer Res. 2013 Jun 1;19(11):2905-16.