![Anti-Myelin Basic Protein antibody [7G7] (ab78156) at 1/10000 dilution + Crude rat spinal cord homogenate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/26444_ab78156WB.jpg)
Anti-Myelin Basic Protein antibody [7G7] (ab78156) at 1/10000 dilution + Crude rat spinal cord homogenate
ab78156 (red), at 1/1000 dilution, staining Myelin Basic Protein in rat mixed neuron/glial cultures. Blue is a DNA stain. ab78156 stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
![Lane 1 : Anti-Myelin Basic Protein antibody [7G7] (ab78156) at 1/10000 dilutionLane 2 : Anti-Myelin Basic Protein antibody [7D2] (ab78157) at 1/10000 dilutionLane 1 : Crude rat spinal cord homogenateLane 2 : Crude rat spinal cord homogenate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/26446_ab78156-3.jpg)
Lane 1 : Anti-Myelin Basic Protein antibody [7G7] (ab78156) at 1/10000 dilutionLane 2 : Anti-Myelin Basic Protein antibody [7D2] (ab78157) at 1/10000 dilutionLane 1 : Crude rat spinal cord homogenateLane 2 : Crude rat spinal cord homogenate
![Overlay histogram showing SH-SY5Y cells stained with ab78156 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78156, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/26447_Myelin-Basic-Protein-Primary-antibodies-ab78156-1.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab78156 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78156, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.