Anti-Myelin Protein Zero antibody
| Name | Anti-Myelin Protein Zero antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31851 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Rat Myelin Protein Zero |
| Description | Rabbit Polyclonal |
| Gene | MPZ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Myelin Protein Zero antibody (ab31851) at 1 µg/ml + Mouse Sciatic Nerve Whole Tissue Lysate at 20 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
ab31851 staining rat cochlear nerve cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 (HBSS with 5% FBS) and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 568 conjugated goat anti-rabbit antibody, diluted 1/2000, was used as the secondary.See Abreview
ab31851 staining Myelin Protein Zero in mouse sciatic nerve tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat. The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.This image is courtesy of an Abreview submitted by Dr Sophie Pezet.
ab31851 staining Myelin Protein Zero in Rat Schwann Cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 5% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/250 in 5% NGS/0.3% Triton X-100 in PBS) for 4 hours at 4°C. A Cy2®-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Mpz - Myelin Protein Zero; Mag - Myelin Associated Glycoprotein; Mbp - Myelin Basic Protein; dCKO - double conditional knockout.See Abreview
ab31851 staining human brainstem sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab31851 at 1/200 in TBS/BSA/azide for 16h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. The myelinated fibres in the rootlet are positive for this antibody (peripheral nerves).See Abreview
Product References
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