Anti-MYH antibody
| Name | Anti-MYH antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55551 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: KLTYQVYGLA LEGQTPVTTV PPGARWLTQE EFHTAAVSTA MKKVFRVYQG QQPGTCMGSK RSQVSSPCSR KKPRMGQQVL DNFFRSHIST DAHSLNSAAQ , corresponding to amino acids 436-536 of Human MYH Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | MUTYH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab55551 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55551, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/26644_MYH-Primary-antibodies-ab55551-1.jpg)
Overlay histogram showing HeLa cells stained with ab55551 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55551, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Genetic instability in lymphoblastoid cell lines expressing biallelic and - Genetic instability in lymphoblastoid cell lines expressing biallelic and
Grasso F, Giacomini E, Sanchez M, Degan P, Gismondi V, Mazzei F, Varesco L, Viel A, Bignami M. Hum Mol Genet. 2014 Jul 15;23(14):3843-52.
MUTYH c.933+3A>C, associated with a severely impaired gene expression, is the - MUTYH c.933+3A>C, associated with a severely impaired gene expression, is the
Pin E, Pastrello C, Tricarico R, Papi L, Quaia M, Fornasarig M, Carnevali I, Oliani C, Fornasin A, Agostini M, Maestro R, Barana D, Aretz S, Genuardi M, Viel A. Int J Cancer. 2013 Mar 1;132(5):1060-9.
Loss of MUTYH function in human cells leads to accumulation of oxidative damage - Loss of MUTYH function in human cells leads to accumulation of oxidative damage
Ruggieri V, Pin E, Russo MT, Barone F, Degan P, Sanchez M, Quaia M, Minoprio A, Turco E, Mazzei F, Viel A, Bignami M. Oncogene. 2013 Sep 19;32(38):4500-8.
Methotrexate induces oxidative DNA damage and is selectively lethal to tumour - Methotrexate induces oxidative DNA damage and is selectively lethal to tumour
Martin SA, McCarthy A, Barber LJ, Burgess DJ, Parry S, Lord CJ, Ashworth A. EMBO Mol Med. 2009 Sep;1(6-7):323-37.