![Overlay histogram showing SH-SH5Y cells stained with ab119701 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119701, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27203_ab119701-2-ab119701FC.jpg)
Overlay histogram showing SH-SH5Y cells stained with ab119701 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119701, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-n-Myc antibody [6A4I] (ab119701) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : Ramos cell lysateLane 3 : HuT78 cell lysateLane 4 : MCF7 cell lysateLane 5 : Mouse Brain tissue lysateLane 6 : Mouse Testis tissue lysateLane 7 : Rat Brain tissue lysateLane 8 : Rat Testis tissue lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27204_n-Myc-Primary-antibodies-ab119701-4.jpg)
All lanes : Anti-n-Myc antibody [6A4I] (ab119701) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : Ramos cell lysateLane 3 : HuT78 cell lysateLane 4 : MCF7 cell lysateLane 5 : Mouse Brain tissue lysateLane 6 : Mouse Testis tissue lysateLane 7 : Rat Brain tissue lysateLane 8 : Rat Testis tissue lysate