Anti-n-Myc antibody [NCM II 100] - ChIP Grade
| Name | Anti-n-Myc antibody [NCM II 100] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab16898 |
| Prices | $395.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | NCM II 100 |
| Applications | IHC-F IP WB ICC/IF ICC/IF FC ChIP |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fusion protein (Human) |
| Description | Mouse Monoclonal |
| Gene | MYCN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunoprecipitation of N-Myc from S35 methionine-labeled IMR-5 cells using ab16898 (lane 1) and Normal Mouse IgG (lane 2). ab16898 used at 1 mg/sample.
![Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27206_n-Myc-Primary-antibodies-ab16898-4.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
BET bromodomain inhibition of MYC-amplified medulloblastoma. - BET bromodomain inhibition of MYC-amplified medulloblastoma.
Bandopadhayay P, Bergthold G, Nguyen B, Schubert S, Gholamin S, Tang Y, Bolin S, Schumacher SE, Zeid R, Masoud S, Yu F, Vue N, Gibson WJ, Paolella BR, Mitra SS, Cheshier SH, Qi J, Liu KW, Wechsler-Reya R, Weiss WA, Swartling FJ, Kieran MW, Bradner JE, Beroukhim R, Cho YJ. Clin Cancer Res. 2014 Feb 15;20(4):912-25.
Target delivery of MYCN siRNA by folate-nanoliposomes delivery system in a - Target delivery of MYCN siRNA by folate-nanoliposomes delivery system in a
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Myc binds the pluripotency factor Utf1 through the basic-helix-loop-helix leucine - Myc binds the pluripotency factor Utf1 through the basic-helix-loop-helix leucine
Laskowski AI, Knoepfler PS. Biochem Biophys Res Commun. 2013 Jun 14;435(4):551-6. doi:
Targeting MYCN in neuroblastoma by BET bromodomain inhibition. - Targeting MYCN in neuroblastoma by BET bromodomain inhibition.
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Multifunctional nanobeacon for imaging Thomsen-Friedenreich antigen-associated - Multifunctional nanobeacon for imaging Thomsen-Friedenreich antigen-associated
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N-Myc and GCN5 regulate significantly overlapping transcriptional programs in - N-Myc and GCN5 regulate significantly overlapping transcriptional programs in
Martinez-Cerdeno V, Lemen JM, Chan V, Wey A, Lin W, Dent SR, Knoepfler PS. PLoS One. 2012;7(6):e39456.
MicroRNA-184 inhibits neuroblastoma cell survival through targeting the - MicroRNA-184 inhibits neuroblastoma cell survival through targeting the
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MicroRNA mediates DNA demethylation events triggered by retinoic acid during - MicroRNA mediates DNA demethylation events triggered by retinoic acid during
Das S, Foley N, Bryan K, Watters KM, Bray I, Murphy DM, Buckley PG, Stallings RL. Cancer Res. 2010 Oct 15;70(20):7874-81.
Global MYCN transcription factor binding analysis in neuroblastoma reveals - Global MYCN transcription factor binding analysis in neuroblastoma reveals
Murphy DM, Buckley PG, Bryan K, Das S, Alcock L, Foley NH, Prenter S, Bray I, Watters KM, Higgins D, Stallings RL. PLoS One. 2009 Dec 4;4(12):e8154.
N-Myc regulates expression of pluripotency genes in neuroblastoma including lif, - N-Myc regulates expression of pluripotency genes in neuroblastoma including lif,
Cotterman R, Knoepfler PS. PLoS One. 2009 Jun 4;4(6):e5799.