Anti-NAK antibody (ab109693) at 2 µg/ml + Jurkat cell lysate at 15 µg
All lanes : Anti-NAK antibody (ab109693) at 1/1000 dilutionLane 1 : 0-4hr old EGFP shRNA embryosLane 2 : 0-4hr old ik2 shRNA embryosSecondarysheep anti-mouse IgG conjugated to HRP at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab109693 at 10µg/ml staining NAK in formalin-fixed, paraffin-embedded Human skin tissue.After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
ab109693 at 10µg/ml staining NAK in formalin-fixed, paraffin-embedded Human testis tissue.After incubation with the primary antibody, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.