TBK1

Gene Symbol TBK1
Entrez Gene 29110
Alt Symbol NAK, T2K
Species Human
Gene Type protein-coding
Description TANK-binding kinase 1
Other Description NF-kB-activating kinase|NF-kappa-B-activating kinase|serine/threonine-protein kinase TBK1
Swissprots Q8IYV3 A8K4S4 Q9NUJ5 Q9UHD2
Accessions EAW97133 Q9UHD2 AA970899 AF174536 AAF69106 AF191838 AAF05989 AK002192 BAA92129 AK291039 BAF83728 AK303683 BAG64676 AW275367 BC034950 AAH34950 DA788357 XM_005268809 XP_005268866 XM_005268810 XP_005268867 XR_944524 XR_944525 NM_013254 NP_037386
Function Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferatio
Subcellular Location Cytoplasm {ECO:0000269|PubMed:15485837, ECO:0000269|PubMed:21813773}. Note=Upon mitogen stimulation or triggering of the immune system, TBK1 is recruited to the exocyst by EXOC2.
Tissue Specificity Ubiquitous with higher expression in testis. Expressed in the ganglion cells, nerve fiber layer and microvasculature of the retina. {ECO:0000269|PubMed:10783893, ECO:0000269|PubMed:21447600}.
Top Pathways Hepatitis C, Toll-like receptor signaling pathway, Measles, Influenza A, Cytosolic DNA-sensing pathway

Phospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb (PE Conjugate) - 13498 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D52C2
Flow cytometric analysis of THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by a day of rest then untreated (blue) or LPS-treated (1 μg/ml, 1 hr; green), using Phospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb (PE Conjugate).

Phospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb - 5483 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D52C2
Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight) followed by treatment with LPS (1 μg/ml), up to 24h, using Phospho-TBK1/NAK (Ser172) (D52C2) XP ® Rabbit mAb (upper), or total TBK1/NAK (D1B4) Rabbit mAb #3504 (lower).

TBK1/NAK (D1B4) Rabbit mAb - 3504 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1B4
Western blot analysis of extracts from various cell lines using TBK1/NAK (D1B4) Rabbit mAb.

TBK1/NAK Antibody - 3013 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa (human), BaF3 (mouse) and KNRK (rat) cell lines, using TBK1/NAK Antibody.

NAK/TBK-1 Antibody - 3012 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

TBK1 (A-6) - sc-398366 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TBK1 (A-6): sc-398366. Western blot analysis of TBK1 expression in RAW 264.7 (A), MCF7 (B) and THP-1 (C) whole cell lysates.

TBK1 (L-15) - sc-9910 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TBK1 (L-15): sc-9910. Western blot analysis of TBK1 expression in RAW 264.7 whole cell lysate.

TBK1 (S-15) - sc-9912 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TBK1 (S-15): sc-9912. Western blot analysis of TBK1 expression in MCF7 whole cell lysate.

TBK1 (M-19) - sc-9911 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

TBK1 (M-375) - sc-9085 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TBK1 (M-375): sc-9085. Western blot analysis of TBK1 expression in non-transfected: sc-117752 (A) and human TBK1 transfected: sc-159010 (B) 293T whole cell lysates.

TBK1 (6D603) - sc-73115 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
TBK1 (6D603): sc-73115. Western blot analysis of TBK1 expression in MCF7 (A), THP-1 (B), NTERA-2 cl.D1 (C) and Jurkat (D) whole cell lysates.

TBK1 (108A429) - sc-52957 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
TBK1 (108A429): sc-52957. Western blot analysis of TBK1 expression in MCF7 (A), THP-1 (B), NTERA-2 cl.D1 (C) and Jurkat (D) whole cell lysates.

Anti-NAK (phospho S172) antibody [EPR2867(2)] - ab109272 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2867(2)
All lanes : Anti-NAK (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/1000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate, treated with Calyculin ALysates/proteins at 10 µg per lane.

Anti-NAK antibody - ab8121 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab8121 at 10µg/ml staining NAK in MOLT4 cells by ICC/IF

Anti-NAK antibody - ab109693 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-NAK antibody (ab109693) at 2 µg/ml + Jurkat cell lysate at 15 µg

Anti-NAK antibody - ab180731 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NAK antibody (ab180731) at 1/500 dilutionLane 1 : BT474 cell line extractLane 2 : U937 cell line extract

Anti-NAK antibody - ab62871 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NAK antibody - ab178528 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-NAK antibody (ab178528) at 1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-NAK antibody [108A429] - ab12116 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 108A429
All lanes : Anti-NAK antibody [108A429] (ab12116) at 2 µg/mlLane 1 : 293 cellsLane 2 : 293 cells transfected with Human NAK cDNA

Anti-NAK antibody [EP611Y] - ab40676 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP611Y
Anti-NAK antibody [EP611Y] (ab40676) at 1/1000 dilution + HeLa cell lysate at 10 µg

Anti-NAK antibody [EPR2867(2)-19] - ab109735 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2867(2)-19
Overlay histogram showing HeLa cells stained with ab109735 (red line) at 1/90 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

Anti-NAK antibody - C-terminal - ab191057 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NAK antibody - C-terminal (ab191057) at 0.5 µg/mlLane 1 : Rat Testis Tissue LysateLane 2 : Rat Liver Tissue Lysate

Anti-NAK / TBK1 Antibody (aa8-35, AP) - LS-C224251 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAK / TBK1 Antibody (aa8-35, Biotin) - LS-C224252 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NAK / TBK1 Antibody (aa8-35, APC) - LS-C224253 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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