Anti-NAK antibody [108A429]
| Name | Anti-NAK antibody [108A429] |
|---|---|
| Supplier | Abcam |
| Catalog | ab12116 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 108A429 |
| Applications | FC WB IP |
| Species Reactivities | Human |
| Antigen | Full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | TBK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-NAK antibody [108A429] (ab12116) at 2 µg/mlLane 1 : 293 cellsLane 2 : 293 cells transfected with Human NAK cDNA](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27372_NAK-Primary-antibodies-ab12116-1.jpg)
All lanes : Anti-NAK antibody [108A429] (ab12116) at 2 µg/mlLane 1 : 293 cellsLane 2 : 293 cells transfected with Human NAK cDNA
![Overlay histogram showing HeLa cells stained with ab12116 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12116, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27373_NAK-Primary-antibodies-ab12116-2.jpg)
Overlay histogram showing HeLa cells stained with ab12116 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12116, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
RING finger protein 11 targets TBK1/IKKi kinases to inhibit antiviral signaling. - RING finger protein 11 targets TBK1/IKKi kinases to inhibit antiviral signaling.
Charoenthongtrakul S, Gao L, Parvatiyar K, Lee D, Harhaj EW. PLoS One. 2013;8(1):e53717.
ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral - ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral
Gao L, Coope H, Grant S, Ma A, Ley SC, Harhaj EW. J Biol Chem. 2011 Oct 21;286(42):36592-602.
TAX1BP1 and A20 inhibit antiviral signaling by targeting TBK1-IKKi kinases. - TAX1BP1 and A20 inhibit antiviral signaling by targeting TBK1-IKKi kinases.
Parvatiyar K, Barber GN, Harhaj EW. J Biol Chem. 2010 May 14;285(20):14999-5009.