Anti-NAK antibody [EPR2867(2)-19]
| Name | Anti-NAK antibody [EPR2867(2)-19] |
|---|---|
| Supplier | Abcam |
| Catalog | ab109735 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR2867(2)-19 |
| Applications | WB IHC-P FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NAK aa 150-250 |
| Description | Rabbit Monoclonal |
| Gene | TBK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab109735 (red line) at 1/90 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.
ab109735 staining NAK in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/800). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.
![Anti-NAK antibody [EPR2867(2)-19] (ab109735) at 1/4000 dilution + HeLa cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27380_ab109735-240446-109735-WB.jpg)
Anti-NAK antibody [EPR2867(2)-19] (ab109735) at 1/4000 dilution + HeLa cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Overlay histogram showing HeLa cells stained with ab109735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109735, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-NAK antibody [EPR2867(2)-19] (ab109735) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : U937 cell lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27382_NAK-Primary-antibodies-ab109735-1.jpg)
All lanes : Anti-NAK antibody [EPR2867(2)-19] (ab109735) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : U937 cell lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109735 at a dilution of 1/250.