Anti-Nanog antibody

• Supplier: Abcam
• Catalog: ab80892
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: FC IHC ICC/IF WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Monkey
• Antigen: Mouse Nanog protein
• Description: Rabbit Polyclonal
• Gene: NANOG
• Conjugate: Unconjugated

Product images

ab80892 staining Nanog in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Tween 20 prior to blocking in 20% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 4 hour at 25°C. A Cy5 conjugated goat polyclonal to rabbit IgG antibody, diluted 1/100, was used as the secondary. Nuclear staining with DAPI.See Abreview

IHC-Wholemount image of anti-Nanog antibody (ab80892) staining on a mouse pre-implantation embryo. This embryo was fixed in paraformaldehyde, permeabilized in 0.25% Triton X and then blocked in 5% serum for 1 hour at room temperature. It was stained with anti-Nanog antibody overnight at 4 degrees (right), and DAPI (left).See Abreview

ab80892 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab80892 at 1/400 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.See Abreview

All lanes : Anti-Nanog antibody (ab80892) at 1/500 dilutionLane 1 : MarkerLane 2 : Mouse embryonic stem cell line D3.Lane 3 : Mouse embryonic stem cell line D3.Lane 4 : Mutant mouse embryonic stem cell line known to not express nanog.Lane 5 : Mutant mouse embryonic stem cell line known to not express nanog.Lysates/proteins at 200000 cells per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Overlay histogram of Nanog staining in mouse embryonic stem cells using ab80892, at 1:100 dilution. Purple histogram represents negative control (rabbit IgG); green line represents anti-Nanog antibody (ab80892).See Abreview

Product References

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