NANOG

Gene Symbol NANOG
Entrez Gene 79923
Alt Symbol -
Species Human
Gene Type protein-coding
Description Nanog homeobox
Other Description homeobox protein NANOG|homeobox transcription factor Nanog|homeobox transcription factor Nanog-delta 48
Swissprots D3DUU4 Q6JZS5 Q2TTG0 Q9H9S0
Accessions AFP90963 AFP90964 AFP90965 AFP90966 AFP90967 AFP90968 AFP90969 AFP90970 AFP90971 AFP90972 AFP90973 AFP90974 AFP90975 AFP90976 AFP90977 AFP90978 AFP90979 AFP90980 AFP90981 AFP90982 AFP90983 AFP90984 AFP90985 AFP90986 AFP90987 AFP90988 AFP90989 AFP90990 AFP90991 AFP90992 AFP90993 AFP90994 AFP90995 AFP90996 AFP90997 AFP90998 AFP90999 AFP91000 AFP91001 AFP91002 AFP91003 AFP91004 AFP91005 AFP91006 AFP91007 AFP91008 AFP91009 AFP91010 AFP91011 AFP91012 AFP91013 AFP91014 AFP91015 AFP91016 AFP91017 AFP91018 AFP91019 AFP91020 AFP91021 AFP91022 AFP91023 AFP91024 AFP91025 AFP91026 AFP91027 AFP91028 AFP91029 AFP91030 AFP91031 AFP91032 AFP91033 AFP91034 AFP91035 AFP91036 AFP91037 AFP91038 AFP91039 AFP91040 AFP91041 AFP91042 AFP91043 AFP91044 AFP91045 AFP91046 AFP91047 AFP91048 AFP91049 AFP91050 AFP91051 AFP91052 AFP91053 AFP91054 AFP91055 AFP91056 CAH25962 EAW88649 EAW88650 EAW88651 Q9H9S0 AB093576 BAC76999 AI656990 AK022643 BAB14151 AK290896 BAF83585 AY230262 AAP49529 AY578089 AAT81526 BC069807 BC098275 BC099704 BC160187 AAI60187 DC425336 EU446847 ABZ92376 XM_011520850 XP_011519152 XM_011520851 XP_011519153 XM_011520852 XP_011519154 NM_001297698 NP_001284627 NM_024865 NP_079141
Function Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. {ECO:0000269|PubMed:15983365, ECO:0000269|PubMed:16000880, ECO:0000269|PubMed:16391521}.
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000269|PubMed:15983365}.
Tissue Specificity Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. {ECO:0000269|PubMed:12787504, ECO:0000269|PubMed:12787505, ECO:0000269|PubMed:14990856, ECO:0000269|PubMed:15982323, ECO:0000269|PubMed:16391521}.
Top Pathways Signaling pathways regulating pluripotency of stem cells, Proteoglycans in cancer

Nanog (D73G4) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) - 8750 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D73G4
Immunofluorescent analysis of NTERA-2 (left) and HeLa (right) cells using Nanog (D73G4) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin (green).

Nanog (D73G4) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 5448 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D73G4
Confocal immunofluorescent analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog (D73G4) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with DY-554 phalloidin (red).

Nanog (D73G4) XP ® Rabbit mAb (ChIP Formulated) - 5232 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 immunoprecipitations)
Host Rabbit
Clonality Monoclonal
Clone D73G4
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NCCIT cells and 10 µl of either Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) #5677 or Nanog (D73G4) XP ® Rabbit mAb (ChIP Formulated), using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5ng enriched ChIP DNA using NEBNext ® Ultra™ II DNA Library Prep Kit for Illumina ® , and sequenced on the Illumina NextSeq. Oct-4 and Nanog are known to associate with each other on chromatin. The figure shows binding of both Oct-4 and Nanog across chromosome 6 (upper), including POU5F1/Oct-4 (lower), a known target gene of both Oct-4 and Nanog (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

Nanog (D73G4) XP ® Rabbit mAb - 4903 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (20 western blots)
Host Rabbit
Clonality Monoclonal
Clone D73G4
Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP ® Rabbit mAb.

Nanog (1E6C4) Mouse mAb - 4893 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1E6C4
Western blot analysis of extracts from NTERA and NCCIT cells using Nanog (1E6C4) Mouse mAb.

StemLight™ Pluripotency Transcription Factor Antibody Kit - 9093 from Cell Signaling Technology

Prices $239.00
Sizes 1 kit (3 x 20 µl)
Clonality Polyclonal
Western blot analysis of extracts from NCCIT, NTERA-2 and iPS cells using Nanog (D73G4) XP ® Rabbit mAb.

StemLight™ Pluripotency Antibody Kit - 9656 from Cell Signaling Technology

Prices $399.00
Sizes 1 kit (100 tests)
Clonality Polyclonal
Confocal immunofluorescent analysis of NTERA-2 cells, untreated (left panel) or retinoic acid-treated (10 µM all-trans RA for 5 days) (right panel), using Neurofilament-L (C28E10) Rabbit mAb #2837 (green, upper), and β3-Tubulin (TU-20) Mouse mAb #4466 (green, lower). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye). Note the appearance of neuronal markers and structures as cells differentiate along the neuronal lineage with retinoic acid treatment.

Nanog Antibody - 3580 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NTERA2 and NCCIT cells using Nanog Antibody.

Nanog (1E6C4) - sc-293121 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nanog (1E6C4): sc-293121. Western blot analysis of Nanog expression in NTERA-2 cl.D1 whole cell lysate.

Nanog (H-2) - sc-374103 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nanog (H-2): sc-374103. Western blot analysis of human recombinant Nanog fusion protein.

Nanog (A-11) - sc-374001 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Nanog (A-11): sc-374001. Western blot analysis of Nanog expression in HeLa nuclear extract.

Nanog (P-18) - sc-30332 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Nanog (H-155) - sc-33759 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Nanog (H-155): sc-33759. Western blot analysis of human recombinant Nanog fusion protein.

Nanog (N-17) - sc-30331 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nanog (N-17): sc-30331. Western blot analysis of human recombinant Nanog.

Nanog (J-29) - sc-81961 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Nanog (J-29): sc-81961. Western blot analysis of Nanog expression in HeLa nuclear extract.

Nanog (5A10) - sc-134218 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
Nanog (5A10): sc-134218. Western blot analysis of Nanog expression in NIH/3T3 whole cell lysate.

Anti-Nanog antibody - ab80892 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab80892 staining Nanog in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Tween 20 prior to blocking in 20% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 4 hour at 25°C. A Cy5 conjugated goat polyclonal to rabbit IgG antibody, diluted 1/100, was used as the secondary. Nuclear staining with DAPI.See Abreview

Anti-Nanog antibody - ab106465 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-Nanog antibody (ab106465) at 1 µg/mlLane 2 : Anti-Nanog antibody (ab106465) at 2 µg/mlLane 1 : Human spleen tissue lysate Lane 2 : Human spleen tissue lysate Lysates/proteins at 15 µg per lane.

Anti-Nanog antibody - ab77095 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-Nanog antibody (ab77095) at 0.1 µg/ml + Human ovary lysate at 35 µgdeveloped using the ECL technique

Anti-Nanog antibody - ab155943 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of Nanog showing staining in the nucleus of paraffin-treated human testicular carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Nanog polyclonal antibody (ab155943) diluted by 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Anti-Nanog antibody - ab131432 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Nanog antibody (ab131432) at 1/500 dilution + whole cell lysate derived form Human embryonic stem cell

Anti-Nanog antibody - ab90168 from Abcam

Prices $384.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
All lanes : Anti-Nanog antibody (ab90168) at 1 µg/mlLane 1 : HUES7 (Human embryonic stem cell line) Whole Cell LysateLane 2 : Testis (Human) Whole Cell Lysate - tumor tissue (ab30258)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Nanog antibody - ab93240 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nanog antibody (ab93240)Lane 1 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell LysateLane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Nanog antibody - ab108487 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Nanog antibody - ab89500 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Nanog antibody (ab89500) at 5 µg/ml + HeLa nuclear extract at 50 µg
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