Anti-Nanog antibody

• Supplier: Abcam
• Catalog: ab70482
• Prices: $398.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB IP
• Species Reactivities: Mouse
• Antigen: A region between residue 250 and the C-terminus (residue 305) of Mouse Nanog using the numbering given in entry NP_079141
• Description: Rabbit Polyclonal
• Gene: Nanog
• Conjugate: Unconjugated

Product images

ab70482 staining Nanog in murine embryonic stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.05% Triton X-100 and blocked using 10% serum for 2 hours at 20°C. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution.See Abreview

All lanes : Anti-Nanog antibody (ab70482) at 0.1 µg/mlLane 1 : Whole cell lysate from mouse embryonic stem cells at 15 µgLane 2 : Whole cell lysate from mouse embryonic stem cells at 50 µgdeveloped using the ECL technique

Detection of Mouse Nanog by Western Blot of Immunoprecipitation with whole cell lysate (1mg) from mouse embryonic stem cells and ab 70482 at 2µg/mg lysate. Lane 1: ab70482, Lane 2: rabbit IgG control Immunoprecipitated Nanog was blotted using an anti-Nanog antibody at 0.1µg/ml.

All lanes : Anti-Nanog antibody (ab70482) at 1/1000 dilution (for 16 hours at 4°C)Lane 1 : Murine Embryonic Fibroblasts (MEFs)Lane 2 : Murine Embryonic Stem (ES) CellsLysates/proteins at 50 µg per lane.SecondaryAn HRP-conjugated Donkey anti-rabbit IgG monoclonal at 1/10000 dilutiondeveloped using the ECL technique

ab70482 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with HIER buffer. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab70482 at 1/750 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.See Abreview

Product References

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