Nanog

Gene Symbol	Nanog
Entrez Gene	71950
Alt Symbol	2410002E02Rik, ENK, ecat4
Species	Mouse
Gene Type	protein-coding
Description	Nanog homeobox
Other Description	ES cell-associated protein 4\|early embryo specific expression NK family\|early embryo specific expression NK-type homeobox protein\|homeobox protein NANOG\|homeobox transcription factor Nanog
Swissprots	Q7TN85 Q80Z64 Q6SMR1 Q5TM83
Accessions	ABD16123 ABD16124 CAH25963 EDK99694 Q5TM83 Q80Z64 AB093574 BAC76998 AB126939 BAD72892 AF507043 AAO67362 AK010332 BAE43219 AK167259 AY278951 AAP92157 AY455282 AAS57554 AY455285 AAS57556 BC083105 BC132017 AAI32018 BC137873 AAI37874 BC144995 AAI44996 BC144996 AAI44997 BY382803 XM_006506651 XP_006506714 XM_011241471 XP_011239773 XM_011241472 XP_011239774 XM_011241473 XP_011239775 NM_001289828 NP_001276757 NM_001289830 NP_001276759 NM_001289831 NP_001276760 NM_028016 NP_082292
Function	Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation (By similarity). {ECO:0000250}.
Subcellular Location	Nucleus {ECO:0000255\|PROSITE- ProRule:PRU00108}.
Top Pathways	Signaling pathways regulating pluripotency of stem cells, Proteoglycans in cancer

Nanog (D2A3) XP ® Rabbit mAb (Mouse Specific) - 8822 from Cell Signaling Technology

Prices	$109.00, $277.00
Sizes	20 µl (2 western blots), 100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D2A3

Western blot analysis of extracts from F9 and murine iPS cells using Nanog (D2A3) XP ® Rabbit mAb (Mouse Specific).

Nanog Antibody (Mouse Specific) - 8600 from Cell Signaling Technology

Prices	$239.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from F9 cells using Nanog Antibody (Mouse Specific).

Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated) - 8785 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (20 immunoprecipitations)
Host	Rabbit
Clonality	Monoclonal
Clone	D1G10

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 F9 cells and either 5 μl of Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Mouse Oct-4 Promoter Primers #4653, SimpleChIP ® Mouse XIST Intron 1 Primers #4659, and SimpleChIP ® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Nanog (C-4) - sc-376915 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Nanog (C-4): sc-376915. Western blot analysis of Nanog expression in F9 whole cell lysate.

Nanog (M-17) - sc-30329 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Nanog (M-17): sc-30329. Western blot analysis of Nanog expression in human (A) and mouse (B) embryonic stem cell lysates. Kindly provided by Dr. Nobuaki Kikyo, Stem Cell Institute, University of Minnesota.

Nanog (M-149) - sc-33760 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Nanog (M-149): sc-33760. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear and cytoplasmic localization.

Nanog (W-18) - sc-30328 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Nanog (W-18): sc-30328. Western blot analysis of Nanog expression in human (A) and mouse (B) embryonic stem cell lysates. Kindly provided by Dr. Nobuaki Kikyo, Stem Cell Institute, University of Minnesota.

Anti-Nanog antibody - ab70482 from Abcam

Prices	$398.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ab70482 staining Nanog in murine embryonic stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.05% Triton X-100 and blocked using 10% serum for 2 hours at 20°C. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution.See Abreview

Anti-Nanog antibody - ab14959 from Abcam

Prices	$398.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Nanog antibody (ab14959) at 1 µg/mlLane 1 : As aboveLane 2 : As aboveLane 3 : Mouse Nanog peptide (ab14960) at 1 µg/mlLane 4 : Mouse Nanog peptide (ab14960) at 1 µg/ml

Anti-Nanog antibody - ab84447 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of Nanog in formalin fixed, paraffin embedded mouse teratoma tissue, using a 1/250 dilution of ab84447. Detection: DAB staining.

Nanog Antibody - NB100-58842 from Novus Biologicals

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Nanog Antibody [NB100-58842] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Nanog Antibody - NB100-588 from Novus Biologicals

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Nanog Antibody [NB100-588] - Detection of Mouse Nanog by Western Blot and Immunoprecipitation.

Nanog Antibody - NBP2-13177 from Novus Biologicals

Prices	$99.00, $279.00
Sizes	25 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry-Paraffin: Nanog Antibody [NBP2-13177] - IHC staining of Nanog in mouse testis using DAB with hematoxylin counterstain.

Nanog Antibody [Unconjugated] - AF2729 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Goat
Clonality	Polyclonal

Nanog Antibody [Biotin] - BAF2729 from Novus Biologicals

Prices	$390.00
Sizes	50 µg
Host	Goat
Clonality	Polyclonal

Nanog Antibody [Alexa Fluor 488] - IC2729G from Novus Biologicals

Prices	$319.00
Sizes	100 tests
Host	Goat
Clonality	Polyclonal

D3 mouse embryonic stem cell line either untreated (dark orange filled histogram) or treated with retinoic acid for 3 days (light orange filled histogram) was stained with Goat Anti-Mouse Nanog Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2729G) or isotype control antibody (Catalog # IC108G, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Nanog Antibody [Allophycocyanin] - IC2729A from Novus Biologicals

Prices	$339.00
Sizes	100 tests
Host	Goat
Clonality	Polyclonal

Nanog Polyclonal Antibody - bs-0829R from Bioss

Prices	$279.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin embedded mouse tooth germ tissue labeled with Anti-Nanog Polyclonal Antibody (bs-0829R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Nanog Polyclonal Antibody - bs-10414R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti-Nanog Polyclonal Antibodyy, Unconjugated (bs-10414R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Nanog Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-0829R-A350 from Bioss

Prices	$379.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Nanog Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-0829R-A488 from Bioss

Prices	$379.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Nanog Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-0829R-A555 from Bioss

Prices	$379.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Nanog Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated - bs-0829R-A647 from Bioss

Prices	$379.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Nanog Polyclonal Antibody, Cy3 Conjugated - bs-0829R-Cy3 from Bioss

Prices	$379.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Nanog Polyclonal Antibody, Cy5 Conjugated - bs-0829R-Cy5 from Bioss

Prices	$379.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Search more