Anti-Nanog antibody
| Name | Anti-Nanog antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab14959 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse |
| Antigen | Synthetic peptide derived from residues 250 to the C-terminus of Mouse Nanog |
| Description | Rabbit Polyclonal |
| Gene | Nanog |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Nanog antibody (ab14959) at 1 µg/mlLane 1 : As aboveLane 2 : As aboveLane 3 : Mouse Nanog peptide (ab14960) at 1 µg/mlLane 4 : Mouse Nanog peptide (ab14960) at 1 µg/ml
All lanes : Anti-Nanog antibody (ab14959) at 0.8 µg/mlLane 1 : ES Cell Nuclear ExtractLane 2 : ES Cell Nuclear Extract
ab14959 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab14959 at 1/600 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.See Abreview
Immunofluorescence analysis of murine B6 ES cells treated with 1000 U/ml LIF and 3 µM CH for 4 days, staining Nanog with ab14959. Cells were fixed in paraformaldehyde, permeabilized with 0.2% Triton X-100 for 30 minutes, and blocked in blocking buffer (PBS containing 5% goat serum). Cells were incubated with primary antibody (1/100) overnight at 4°C, followed by a Cy3®-conjugated anti-rabbit secondary antibody,
Product References
Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes - Pleiotropy of glycogen synthase kinase-3 inhibition by CHIR99021 promotes
Ye S, Tan L, Yang R, Fang B, Qu S, Schulze EN, Song H, Ying Q, Li P. PLoS One. 2012;7(4):e35892.
The developmental dismantling of pluripotency is reversed by ectopic Oct4 - The developmental dismantling of pluripotency is reversed by ectopic Oct4
Osorno R, Tsakiridis A, Wong F, Cambray N, Economou C, Wilkie R, Blin G, Scotting PJ, Chambers I, Wilson V. Development. 2012 Jul;139(13):2288-98.
Caspase activity mediates the differentiation of embryonic stem cells. - Caspase activity mediates the differentiation of embryonic stem cells.
Fujita J, Crane AM, Souza MK, Dejosez M, Kyba M, Flavell RA, Thomson JA, Zwaka TP. Cell Stem Cell. 2008 Jun 5;2(6):595-601.