All lanes : Anti-Nanog antibody (ab90168) at 1 µg/mlLane 1 : HUES7 (Human embryonic stem cell line) Whole Cell LysateLane 2 : Testis (Human) Whole Cell Lysate - tumor tissue (ab30258)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of ab90168 staining in human testis seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90168, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Nanog antibody (ab90168) at 1 µg/ml + Human Nanog full length protein (ab50053) at 0.1 µgSecondaryGoat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.