Anti-Nanos3 antibody
| Name | Anti-Nanos3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70001 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic 16 amino acid peptide near the carboxy terminus of the human Nanos3 (GenBank accession no |
| Blocking Peptide | Nanos3 peptide |
| Description | Rabbit Polyclonal |
| Gene | NANOS3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Nanos3 antibody (ab70001) at 2 µg/ml + Human brain tissue lysate at 15 µg
ab70001 at 2.5µg/ml staining Nanos3 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
ICC/IF image of ab70001 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70001, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of Nanos3 in Human Brain cells using ab70001 at 20 ug/ml.
Product References
Activation of the germ-cell potential of human bone marrow-derived cells by a - Activation of the germ-cell potential of human bone marrow-derived cells by a
Liu C, Ma Z, Xu S, Hou J, Hu Y, Yu Y, Liu R, Chen Z, Lu Y. Sci Rep. 2014 Jul 7;4:5564.
A NANOS3 mutation linked to protein degradation causes premature ovarian - A NANOS3 mutation linked to protein degradation causes premature ovarian
Wu X, Wang B, Dong Z, Zhou S, Liu Z, Shi G, Cao Y, Xu Y. Cell Death Dis. 2013 Oct 3;4:e825.
Analysis of meiosis regulators in human gonads: a sexually dimorphic - Analysis of meiosis regulators in human gonads: a sexually dimorphic
Jorgensen A, Nielsen JE, Blomberg Jensen M, Graem N, Rajpert-De Meyts E. Mol Hum Reprod. 2012 Nov;18(11):523-34.
Spontaneous germline potential of human hepatic cell line in vitro. - Spontaneous germline potential of human hepatic cell line in vitro.
Ma Z, Liu R, Wang X, Huang M, Gao Q, Lu Y, Liu C. Mol Hum Reprod. 2013 Apr;19(4):216-26.
NANOS3 function in human germ cell development. - NANOS3 function in human germ cell development.
Julaton VT, Reijo Pera RA. Hum Mol Genet. 2011 Jun 1;20(11):2238-50.