Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
Flow cytometry analysis of HeLa cells using ab181169 at a 1/40 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/40 dilution.
Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling NASP with ab181169 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
All lanes : Anti-NASP antibody [EPR12827] (ab181169) at 1/10000 dilutionLane 1 : HeLa lysateLane 2 : Caco2 lysateLane 3 : 293 lysateLane 4 : NTERA2/D1 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution