NASP

Gene Symbol NASP
Entrez Gene 4678
Alt Symbol FLB7527, PRO1999
Species Human
Gene Type protein-coding
Description nuclear autoantigenic sperm protein (histone-binding)
Other Description histone H1-binding protein|nuclear autoantigenic sperm protein
Swissprots Q96A69 Q5T625 D3DQ07 A8K6H2 Q9BTW2 Q5T622 Q53GW5 F5H3J2 B4DQP3 P49321
Accessions EAX06964 EAX06965 EAX06966 EAX06967 EAX06968 EAX06969 P49321 AF035191 AAD02006 AK056161 AK092829 AK131249 AK222778 BAD96498 AK222816 BAD96536 AK291637 BAF84326 AK298893 BAG61005 AK299584 BAG61519 AK308001 AL512718 AL571577 AY700118 BC003113 AAH03113 BC009933 AAH09933 BC010105 AAH10105 BC011580 AAH11580 BC053608 AAH53608 BT006757 AAP35403 CK300084 CX781565 DC361634 DQ893275 ABM84201 DQ896606 ABM87605 M97856 AAA36027 XM_005270888 XP_005270945 XM_005270889 XP_005270946 XM_011541509 XP_011539811 NM_001195193 NP_001182122 NM_002482 NP_002473 NM_152298 NP_689511 NM_172164
Function Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus.
Tissue Specificity Isoform 1 is testis- and sperm-specific.

NASP (A-6) - sc-398379 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NASP (A-6): sc-398379. Western blot analysis of NASP expression in NTERA-2 cl.D1 (A) and HeLa (B) whole cell lysates.

NASP (S-16) - sc-161915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NASP (S-16): sc-161915. Western blot analysis of NASP expression in NTERA-2 cl.D1 (A), HeLa (B), HL-60 (C) and Caco-2 (D) whole cell lysates.

NASP (E-2) - sc-398102 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NASP (E-2): sc-398102. Western blot analysis of NASP expression in NTERA-2 cl.D1 (A) and HeLa (B) whole cell lysates.

NASP (T-15) - sc-161916 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NASP (H-300) - sc-367193 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NASP (H-300): sc-367193. Western blot analysis of NASP expression in non-transfected 293T: sc-117752 (A), mouse NASP transfected 293T: sc-125689 (B) and NTERA-2 cl.D1 (C) whole cell lysates.

NASP (E-14) - sc-161914 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NASP (E-14): sc-161914. Western blot analysis of NASP expression in NTERA-2 cl.D1 whole cell lysate.

Anti-NASP antibody - ab104233 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NASP antibody (ab104233) at 1 µg/mlLane 1 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NASP antibody - ab72494 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NASP antibody (ab72494) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µg

Anti-NASP antibody [EPR12827] - ab181169 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12827
Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.

Anti-NASP Antibody (aa152-180, AP) - LS-C207305 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NASP Antibody (aa152-180, APC) - LS-C207306 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NASP Antibody (aa152-180, Biotin) - LS-C207307 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NASP Antibody (aa152-180, FITC) - LS-C207308 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NASP Antibody (aa152-180, Azide-free, HRP) - LS-C207310 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NASP Antibody (aa160-190) - LS-C323234 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NASP Antibody (aa150-200) - LS-C287841 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human NASP by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-NASP antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-NASP Antibody (aa152-180, PE) - LS-C207311 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NASP Antibody (aa152-180) - LS-C164750 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of NASP (arrow) using rabbit polyclonal NASP Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NASP gene (Lane 2).

Anti-NASP Antibody (aa287-336) IHC-plus™ - LS-B5873 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NASP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5873 concentration 5 ug/ml.

NASP Antibody - NBP2-33910 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NASP Antibody [NBP2-33910] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

NASP Antibody - NBP2-33929 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: NASP Antibody [NBP2-33929] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.

NASP Antibody - NBP1-52914 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NASP Antibody [NBP1-52914] - Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1 : 312500 Positive control: HepG2 cell lysate NASP is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.

NASP Antibody - NBP2-33928 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NASP Antibody [NBP2-33928] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Negative control (vector only transfected HEK293T lysate)<br/>Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400884)

Anti-NASP antibody produced in rabbit - SAB2104619 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NASP: <B>Cat. No. SAB2104619</B>: Immunohistochemistry of NASP in Testis tissue with NASP antibody at 5.0 μg/mL.

ANTI-NASP(N-TERMINAL) antibody produced in rabbit - SAB1306962 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-NASP (N-term): <B>Cat. No. SAB1306962</B>: Western blot analysis of NASP Antibody (arrow) using Rabbit polyclonal NASP (N-term) . 293 cell lysates (2 μg).<BR/><B>Lanes:</B><BR/>1. Nontransfected with the NASP gene<BR/>2. Transiently transfected with the NASP gene
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