ab76842 staining FLJ37478 in CHO cells transfected with mouse Nat8l cDNA by Immunocytochemistry/ Immunoflourescence. Cells were PFA-fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/150 in 1% BSA in PBS and incubated with the sample for 1 hour at 25°C. The secondary antibody was Cy2®-conjugated Goat- anti-Rabbit polyclonal, diluted 1/200.See Abreview
All lanes : Anti-NAT8L antibody (ab76842) at 1/1000 dilution (in TBS + 0.05% Tween 20 for 1 hour at 22°C)Lane 1 : CHO-K1 cells expressing mouse Nat8L cDNALane 2 : CHO-K1 cells transfected with empty vectorLysates/proteins at 70 µg per lane.SecondaryAn HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.