Anti-Nav1.7 antibody
| Name | Anti-Nav1.7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab65167 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P IHC-F WB IHC-F |
| Species Reactivities | Mouse, Human, Rat |
| Antigen | Synthetic peptide within Human Nav1 |
| Blocking Peptide | Nav1.7 peptide |
| Description | Rabbit Polyclonal |
| Gene | SCN9A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab65167 staining Nav1.7 in human sperm cells by Immunocytochemistry/ Immunofluorescence.Sperm cells were washed, re-suspended in phosphate-buffered saline and smeared onto poly-L-lysine-coated slides. Spermatozoa were then fixed by incubation in cold methanol (-20°C) for 20 minutes. Slides were washed three times for 10 minutes with PBS and incubated with 2% BSA in PBS for 30 minutes to block non-specific sites. Test slides were incubated with ab65167 at a 1/100 dilution. Samples were washed three times in PBS, and incubated for 60 minutes with appropriate FITC-conjugated secondary antibodies. Slides were further washed in PBS, mounted using Vectashield and examined with a Olympus BX-51 fluorescence microscopy using a 100× immersion objective.
ICC/IF image of ab65167 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65167, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of rat dorsal root ganglia, staining Nav1.7 with ab65167. Tissue was blocked with 1% BSA and permeabilized with 0.01% Triton X-100, before incubating with primary antibody (1/500 in PBS with 0.1% BSA + 0.01% Triton X-100) was overnight at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody.
Product References
Candidate hippocampal biomarkers of susceptibility and resilience to stress in a - Candidate hippocampal biomarkers of susceptibility and resilience to stress in a
Henningsen K, Palmfeldt J, Christiansen S, Baiges I, Bak S, Jensen ON, Gregersen N, Wiborg O. Mol Cell Proteomics. 2012 Jul;11(7):M111.016428.
Loss-of-function mutations in sodium channel Nav1.7 cause anosmia. - Loss-of-function mutations in sodium channel Nav1.7 cause anosmia.
Weiss J, Pyrski M, Jacobi E, Bufe B, Willnecker V, Schick B, Zizzari P, Gossage SJ, Greer CA, Leinders-Zufall T, Woods CG, Wood JN, Zufall F. Nature. 2011 Apr 14;472(7342):186-90.