![All lanes : Anti-Nav1.7 antibody [mAbcam62758] (ab62758) at 1 µg/mlLane 1 : Spinal Cord (Mouse) Tissue Lysate Lane 2 : Spinal Cord (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27805_Nav17-Primary-antibodies-ab62758-1.jpg)
All lanes : Anti-Nav1.7 antibody [mAbcam62758] (ab62758) at 1 µg/mlLane 1 : Spinal Cord (Mouse) Tissue Lysate Lane 2 : Spinal Cord (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab62758 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62758, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.