Immunohistochemical analysis of Mouse brain tissue labeling Nav1.7 with ab85015 at 1μg/ml.
![All lanes : Anti-Nav1.7 antibody [S68-6] (ab85015) at 1 µg/mlLane 1 : Molecular weight markerLane 2 : Cell lysates prepared from CHO cell line constitutively expressing Nav1.7 at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27808_Nav17-Primary-antibodies-ab85015-1.jpg)
All lanes : Anti-Nav1.7 antibody [S68-6] (ab85015) at 1 µg/mlLane 1 : Molecular weight markerLane 2 : Cell lysates prepared from CHO cell line constitutively expressing Nav1.7 at 10 µg
ab85015 at 1/100 dilution staining Nav1.7 in mouse back skin tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used. Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. A Fluorophore conjugated goat anti mouse at 1/50 dilution was used as secondary.
![Overlay histogram showing SH-SY5Y cells stained with ab85015 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85015, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27810_Nav17-Primary-antibodies-ab85015-6.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab85015 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85015, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.