Anti-NBR1 antibody
| Name | Anti-NBR1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55474 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IP IHC-P FC |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment: EPQVTLNVTF KNEIQSFLVS DPENTTWADI EAMVKVSFDL NTIQIKYLDE ENEEVSINSQ GEYEEALKMA VKQGNQLQMQ VHEGHHVVDE APPPV, corresponding to amino acids 2-97 of Human NBR1 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | NBR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab55474 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55474, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27849_ab55474-3-ab55474FC.jpg)
Overlay histogram showing HeLa cells stained with ab55474 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55474, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot against tagged recombinant protein immunogen using ab55474 NBR1 antibody at 1ug/ml.
ab55474 (4µg/ml) staining NBR1 in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is moderate cytoplasmic staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
Novel binding partners and differentially regulated phosphorylation sites clarify - Novel binding partners and differentially regulated phosphorylation sites clarify
Cunningham DL, Creese AJ, Auciello G, Sweet SM, Tatar T, Rappoport JZ, Grant MM, Heath JK. PLoS One. 2013 Apr 23;8(4):e61513.
The ubiquitin ligase parkin mediates resistance to intracellular pathogens. - The ubiquitin ligase parkin mediates resistance to intracellular pathogens.
Manzanillo PS, Ayres JS, Watson RO, Collins AC, Souza G, Rae CS, Schneider DS, Nakamura K, Shiloh MU, Cox JS. Nature. 2013 Sep 26;501(7468):512-6.
MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates - MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates
Marchbank K, Waters S, Roberts RG, Solomon E, Whitehouse CA. Int J Cell Biol. 2012;2012:208014.
Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, - Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1,
Perera S, Holt MR, Mankoo BS, Gautel M. Dev Biol. 2011 Mar 1;351(1):46-61.
Neighbor of Brca1 gene (Nbr1) functions as a negative regulator of postnatal - Neighbor of Brca1 gene (Nbr1) functions as a negative regulator of postnatal
Whitehouse CA, Waters S, Marchbank K, Horner A, McGowan NW, Jovanovic JV, Xavier GM, Kashima TG, Cobourne MT, Richards GO, Sharpe PT, Skerry TM, Grigoriadis AE, Solomon E. Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):12913-8. doi: