ab74095 at 1/50 dilution staining NCF1 in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
ab74095 at 1/500 dilution staining NCF1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
All lanes : Anti-NCF1 (phospho S359) antibody (ab74095) at 1/500 dilutionLane 1 : HeLa cell extracts treated with nocodazole (1ug/ml, 18hours)Lane 2 : HeLa cell extracts treated with TSA (400nM, 24hours)Lane 3 : HeLa cell extracts treated with nocodazole (1ug/ml, 18hours) and with TSA (400nM, 24hours) with the immunising peptide at 10 µgLysates/proteins at 30 µg per lane.