NCF1

Gene Symbol NCF1
Entrez Gene 653361
Alt Symbol NCF1A, NOXO2, SH3PXD1A, p47phox
Species Human
Gene Type protein-coding
Description neutrophil cytosolic factor 1
Other Description 47 kDa autosomal chronic granulomatous disease protein|47 kDa neutrophil oxidase factor|NADPH oxidase organizer 2|NCF-1|NCF-47K|SH3 and PX domain-containing protein 1A|neutrophil NADPH oxidase factor 1|neutrophil cytosol factor 1|neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)|nox organizer 2|nox-organizing protein 2|p47-phox
Swissprots Q9UMU2 Q2PP07 A8K7S9 Q53FR5 Q9BXI8 A6NEH2 Q9UDV9 Q9BU90 O43842 Q9BXI7 P14598
Accessions AAA60086 AAA60087 AAB95193 AAF34737 AAS07465 P14598 AF330625 AAK19516 AF330626 AAK19517 AK127905 BAG54596 AK223217 BAD96937 AK292094 BAF84783 AK316341 BAH14712 AK316552 BAG65630 AW204865 AW207763 BC002816 AAH02816 BC065731 AAH65731 DQ893150 ABM84076 DQ896445 ABM87444 GU215077 HQ447816 ADQ32300 U25793 AAA93232 XM_005250543 XP_005250600 XM_005250544 XP_005250601 XM_011516498 XP_011514800 XM_011516499 XP_011514801 XM_011516500 XP_011514802 XM_011516501 XP_011514803 XR_242262 XR_927515 NM_000265 NP_000256
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). {ECO:0000269|PubMed:19801500, ECO:0000269|PubMed:2547247, ECO:0000269|PubMed:2550933}.
Subcellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity Detected in peripheral blood monocytes and neutrophils (at protein level). {ECO:0000269|PubMed:2547247, ECO:0000269|PubMed:2550933}.
Top Pathways Leukocyte transendothelial migration, Fc gamma R-mediated phagocytosis, Chemokine signaling pathway, Leishmaniasis, Phagosome

p47phox Antibody - 4312 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of cell extracts from various cell types using p47phox Antibody.

p47phox (D21F6) Rabbit mAb - 4301 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D21F6
Western blot analysis of extracts from Ramos and RL cells using p47phox (D21F6) Rabbit mAb.

p47-phox (A-7) - sc-17844 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p47-phox (A-7): sc-17844. Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear and cytoplasmic staining of follicle and non-follicle cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

p47-phox (C-20) - sc-7660 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p47-phox (C-20): sc-7660. Western blot analysis of p47-phox expression in whole cell lysates prepared from control (A) and DMSO-treated (B) HL-60 cells.

p47-phox (D-10) - sc-17845 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p47-phox (D-10): sc-17845. Western blot analysis of p47-phox expression in HL-60 (A), THP-1 (B), RAW 264.7 (C) and untreated J774A.1 (D) whole cell lysates.

p47-phox (F-15) - sc-23492 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p47-phox (F-15): sc-23492. Western blot analysis of p47-phox expression in HT-29 (A), THP-1 (B) and U-937 (C) whole cell lysates.

p47-phox (H-195) - sc-14015 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p47-phox (H-195): sc-14015. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.

Anti-NCF1 (phospho S304) antibody - ab63554 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NCF1 (phospho S304) antibody (ab63554) at 1/500 dilutionLane 1 : extracts from COS7 cells treated with UV (15mins)Lane 2 : A549 cells extractsLane 3 : A549 cells extracts with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-NCF1 (phospho S328) antibody - ab111855 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111855 at 1/50 dilution staining NCF1 in paraffin-embedded Human Lymph node tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-NCF1 (phospho S359) antibody - ab74095 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab74095 at 1/50 dilution staining NCF1 in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-NCF1 (phospho S359) antibody - ab195735 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NCF1 (phospho S359) antibody (ab195735) at 1/500 dilutionLane 1 : Nocodazole treated HeLa whole cell lysate with antigen-specific peptideLane 2 : Nocodazole treated HeLa whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-NCF1 antibody - ab795 from Abcam

Prices $382.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-NCF1 antibody (ab795) at 1/0.5 dilution + U937 lysate in RIPA buffer. at 35 µg

Anti-NCF1 antibody - ab63361 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NCF1 antibody (ab63361) at 1/500 dilutionLane 1 : extracts from COS7 cells, treated with UV (15mins)Lane 2 : extracts from COS7 cells, treated with UV (15mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-NCF1 antibody - ab115564 from Abcam

Prices $378.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-NCF1 antibody (ab115564) at 0.2 µg/ml + U937 lysate (in RIPA buffer) at 30 µgdeveloped using the ECL technique

Anti-NCF1 antibody - ab166930 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-NCF1 antibody (ab166930) at 0.03 µg/ml + Mouse Thymus lysates at 35 µgdeveloped using the ECL technique

Anti-NCF1 antibody - ab137950 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NCF1 antibody (ab137950) at 1/500 dilution + COLO205 cell lysate at 30 µg

Anti-NCF1 antibody - ab176483 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Transglutaminase 2 antibody (ab176463) at 1/1000 dilutionLane 1 : Recombinant His-tagged NCF1 proteinLane 2 : RAW 264.7 cell lysatedeveloped using the ECL technique

Anti-NCF1 antibody - ab199640 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NCF1 antibody (ab199640) at 1/250 dilutionLane 1 : Extracts from HepG2 cellsLane 2 : Extracts from Jurkat cellsLysates/proteins at 12.5 µg per lane.

Anti-NCF1 antibody - ab107125 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NCF1 antibody (ab107125) at 1/100 dilution + Ramos cell line lysates at 35 µg

Anti-NCF1 antibody [EPR13134] - ab179457 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13134
All lanes : Anti-NCF1 antibody [EPR13134] (ab179457) at 1/1000 dilutionLane 1 : Raji cell lysateLane 2 : Ramos cell lysateLane 3 : THP1 cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labeled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique

Anti-NCF1 antibody [EPR13134-23] - ab181090 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13134-23
Western blot analysis of Raji cell lysate immunoprecipitated with ab181090 at 1/20 dilution (Lane 1). Lane 2: Negative control.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Anti-NCF1 / p47phox / p47 phox Antibody (aa331-380) - LS-C118013 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human colon carcinoma tissue, using p47 phox (Ab-359) Antibody. The picture on the right is treated with the synthesized peptide.

Anti-NCF1 / p47phox / p47 phox Antibody - LS-C136935 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NCF1 / p47phox / p47 phox Antibody (aa311-360) - LS-C118369 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human brain tissue, using p47 phox (Ab-345) Antibody. The picture on the right is treated with the synthesized peptide.

Anti-NCF1 / p47phox / p47 phox Antibody (aa341-390) IHC-plus™ - LS-B5698 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-p47phox antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5698 concentration 5 ug/ml.
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