Anti-NCF4 antibody [EP2142Y]
| Name | Anti-NCF4 antibody [EP2142Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab76158 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP2142Y |
| Applications | WB IP IHC-P ICC/IF FC |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to residues near the C terminus of human NCF4 |
| Description | Rabbit Monoclonal |
| Gene | NCF4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab76158 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76158, 1/25 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab76158 at 1/100-1/250 dilution staining NCF4 in human spleen by Immunohistochemistry, Paraffin-embedded tissue.
![Anti-NCF4 antibody [EP2142Y] (ab76158) at 1/50000 dilution + HL60 cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/28009_ab76158wb.gif)
Anti-NCF4 antibody [EP2142Y] (ab76158) at 1/50000 dilution + HL60 cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution
Overlay histogram showing K562 cells stained with ab76158 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76158, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Endotoxin priming of neutrophils requires endocytosis and NADPH oxidase-dependent - Endotoxin priming of neutrophils requires endocytosis and NADPH oxidase-dependent
Lamb FS, Hook JS, Hilkin BM, Huber JN, Volk AP, Moreland JG. J Biol Chem. 2012 Apr 6;287(15):12395-404.
Cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation - Cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation
Ueyama T, Nakakita J, Nakamura T, Kobayashi T, Kobayashi T, Son J, Sakuma M, Sakaguchi H, Leto TL, Saito N. J Biol Chem. 2011 Nov 25;286(47):40693-705.
Multiple mechanisms of NADPH oxidase inhibition by type A and type B Francisella - Multiple mechanisms of NADPH oxidase inhibition by type A and type B Francisella
McCaffrey RL, Schwartz JT, Lindemann SR, Moreland JG, Buchan BW, Jones BD, Allen LA. J Leukoc Biol. 2010 Oct;88(4):791-805.