NCF4

Gene Symbol NCF4
Entrez Gene 4689
Alt Symbol NCF, P40PHOX, SH3PXD4
Species Human
Gene Type protein-coding
Description neutrophil cytosolic factor 4, 40kDa
Other Description NCF-4|SH3 and PX domain-containing protein 4|neutrophil NADPH oxidase factor 4|neutrophil cytosol factor 4|p40-phox
Swissprots Q86U56 Q15080 Q9BU98 Q9NP45 O60808 A8K4F9
Accessions AAB39970 ABC40739 EAW60122 EAW60123 EAW60124 Q15080 AB025219 BAA89791 AB025220 BAA89792 AK223324 BAD97044 AK290924 BAF83613 BC002798 AAH02798 BT007346 AAP36010 BU076892 BX355636 CR456528 CAG30414 CR542078 CAG46875 CU013128 CAK54559 CU013416 CAK54858 X77094 CAA54372 XM_011530198 XP_011528500 XM_011530199 XP_011528501 NM_000631 NP_000622 NM_013416 NP_038202
Function Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex. {ECO:0000269|PubMed:8280052}.
Subcellular Location Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Membrane; Peripheral membrane protein.
Tissue Specificity Expression is restricted to hematopoietic cells.
Top Pathways Leukocyte transendothelial migration, Leishmaniasis, Phagosome, Osteoclast differentiation

Phospho-p40phox (Thr154) Antibody - 4311 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from THP1 cells, untreated or PMA-treated, and guinea pig neutrophils, untreated or fMLP-treated, using Phospho-p40phox (Thr154) Antibody.

p40-phox (N-20) - sc-18252 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p40-phox (N-20): sc-18252. Western blot analysis of p40-phox expression in RAW 264.7 whole cell lysate.

p40-phox (H-300) - sc-30087 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p40-phox (H-300): sc-30087. Western blot analysis of p40-phox expression in RAW 264.7 whole cell lysate.

p40-phox (E-20) - sc-18253 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p40-phox (E-20): sc-18253. Western blot analysis of p40-phox expression in non-transfected: sc-117752 (A) and human p40-phox transfected: sc-129386 (B) 293T whole cell lysates.

p40-phox (D-8) - sc-48388 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p40-phox (D-8): sc-48388. Western blot analysis of p40-phox expression in human peripheral blood (A) and HL-60 (B) whole cell lysates.

p40-phox (B-1) - sc-48376 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p40-phox (B-1): sc-48376. Western blot analysis of p40-phox expression in human peripheral blood (A) and HL-60 (B) whole cell lysate.

p-p40-phox (B-8) - sc-377550 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of p40-phox phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) THP-1 whole cell lysates. Antibodies tested include p-p40-phox (B-8): sc-377550 (A,B) and p40-phox (B-1): sc-48376 (C,D).

p-p40-phox (Thr 154) - sc-33403 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of p40-phox phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) THP-1 whole cell lysates. Antibodies tested include p-p40-phox (Thr 154): sc-33403 (A,B) and p40-phox (B-1): sc-48376 (C,D).

Anti-NCF4 (phospho T154) antibody - ab59355 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59355, at a 1/50 dilution, staining human NCF4 in ovary, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).

Anti-NCF4 antibody - ab85259 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NCF4 antibody (ab85259) at 1/50 dilution + K-562 cell line lysates at 35 µg

Anti-NCF4 antibody - ab89646 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-NCF4 antibody (ab89646) at 1 µg/ml + Human liver tissue lysate at 50 µg

Anti-NCF4 antibody [4H363] - ab14977 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4H363

Anti-NCF4 antibody [EP2142Y] - ab76158 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2142Y
ICC/IF image of ab76158 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76158, 1/25 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-P40PHOX / NCF4 Antibody - LS-C9417 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal

Anti-P40PHOX / NCF4 Antibody - LS-C9418 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-P40PHOX / NCF4 Antibody (C-Terminus) - LS-C9419 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-P40PHOX / NCF4 Antibody (aa120-169) - LS-C117767 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-P40PHOX / NCF4 Antibody (C-Terminus, clone EP2142Y) - LS-C50073 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2142Y
IHC of paraffin-embedded spleen tissue using anti-p40 antibody.

Anti-P40PHOX / NCF4 Antibody (aa216-265) - LS-C102201 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NCF4 antibody LS-C102201 Western blot of HepG2 cell lysate.

Anti-P40PHOX / NCF4 Antibody (aa51-100) - LS-C80786 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal Thymus cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.

Anti-P40PHOX / NCF4 Antibody - LS-C152787 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
WB: RAW 264.7 cell lysate was probed with anti-p40-phox (0.5g/ml).

Anti-P40PHOX / NCF4 Antibody (aa267-316) - LS-C117085 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NCF4 antibody LS-C117085 Western blot of Fetal Muscle lysate.

Anti-P40PHOX / NCF4 Antibody - LS-C192845 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of K562 cell lines, using NCF4 antibody.

Anti-P40PHOX / NCF4 Antibody - LS-C155298 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 10% SDS PAGE NCF4 antibody diluted at 1:1000

Anti-P40PHOX / NCF4 Antibody (phospho-Thr154) - LS-C199374 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human ovary, using p40 phox (Phospho-Thr154) Antibody. The sample on the right was incubated with synthetic peptide.
Search more