Anti-Nck antibody [Y531]
| Name | Anti-Nck antibody [Y531] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32120 |
| Prices | $391.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y531 |
| Applications | WB IHC-P ICC/IF FC IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues close to the SH2 domain of human Nck |
| Description | Rabbit Monoclonal |
| Gene | NCK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing Jurkat cells stained with ab32120 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32120, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-Nck antibody [Y531] (ab32120) at 1/10000 dilution + Hela cell lysateObserved band size : 47 kDa (why is the actual band size different from the predicted?)Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/28027_ab32120-1.JPG)
Anti-Nck antibody [Y531] (ab32120) at 1/10000 dilution + Hela cell lysateObserved band size : 47 kDa (why is the actual band size different from the predicted?)Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
ab32120 (1/100), staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue.
Product References
Activation of diverse signalling pathways by oncogenic PIK3CA mutations. - Activation of diverse signalling pathways by oncogenic PIK3CA mutations.
Wu X, Renuse S, Sahasrabuddhe NA, Zahari MS, Chaerkady R, Kim MS, Nirujogi RS, Mohseni M, Kumar P, Raju R, Zhong J, Yang J, Neiswinger J, Jeong JS, Newman R, Powers MA, Somani BL, Gabrielson E, Sukumar S, Stearns V, Qian J, Zhu H, Vogelstein B, Park BH, Pandey A. Nat Commun. 2014 Sep 23;5:4961.
A remote cis-acting variant at 3q links glomerular NCK1 to diabetic nephropathy. - A remote cis-acting variant at 3q links glomerular NCK1 to diabetic nephropathy.
He B, Osterholm AM, Ojala JR, Andersson AC, Tryggvason K. PLoS One. 2013;8(2):e56414.
Once phosphorylated, tyrosines in carboxyl terminus of protein-tyrosine kinase - Once phosphorylated, tyrosines in carboxyl terminus of protein-tyrosine kinase
de Castro RO, Zhang J, Groves JR, Barbu EA, Siraganian RP. J Biol Chem. 2012 Mar 9;287(11):8194-204.