NCK1

Gene Symbol NCK1
Entrez Gene 4690
Alt Symbol NCK, NCKalpha, nck-1
Species Human
Gene Type protein-coding
Description NCK adaptor protein 1
Other Description NCK tyrosine kinase|SH2/SH3 adaptor protein NCK-alpha|cytoplasmic protein NCK1|melanoma NCK protein|non-catalytic region of tyrosine kinase
Swissprots D3DNE3 P16333 B7Z751
Accessions EAW79105 EAW79106 EAW79107 EAW79108 EAW79109 P16333 AK095534 AK301460 BAH13487 AK312692 BAG35571 BC006403 AAH06403 BP339594 DA265476 DA855124 DB117533 DQ893094 ABM84020 EU832672 ACE87763 EU832751 ACE87083 X17576 CAA35599 NM_001190796 NP_001177725 NM_001291999 NP_001278928 NM_006153 NP_006144
Function Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors. {ECO:0000269|PubMed:10026169, ECO:0000269|PubMed:16835242, ECO:0000269|PubMed:17803907, ECO:0000269|PubMed:18835251, ECO:0000269|PubMed:23358419, ECO:0000269|PubMed:9430661}.
Subcellular Location Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
Top Pathways Axon guidance, T cell receptor signaling pathway, Pathogenic Escherichia coli infection, ErbB signaling pathway

NCK1 (5B7) Mouse mAb - 12778 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 5B7
Western blot analysis of extracts from various cell lines using NCK1 (5B7) Mouse mAb.

NCK1 (15B9) Rabbit mAb - 2319 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 15B9
Western blot analysis of total cell lysates from 293, A10, NIH/3T3 and COS cells, using NCK1 (15B9) Rabbit mAb.

NCK1 (20B.1H9) - sc-20026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCK1 (20B.1H9): sc-20026. Western blot analysis of NCK1 expression in H4 cell extracts.

NCK1 (S-15) - sc-22442 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NCK1 (108) - sc-136232 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
NCK1 (108): sc-136232. Immunofluorescence staining of HISM cells showing cytoplasmic localization.

NCK1/2 (G-3) - sc-365802 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCK1/2 (G-3): sc-365802. Western blot analysis of NCK1 expression in non-transfected: sc-117752 (A) and mouse NCK1 transfected: sc-121953 (B) 293T whole cell lysates.

NCK1/2 (H-300) - sc-50365 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NCK1/2 (H-300): sc-50365. Western blot analysis of NCK1 expression in non-transfected: sc-117752 (A) and mouse NCK1 transfected: sc-121953 (B) 293T whole cell lysates.

NCK1/2 (C-19) - sc-290 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NCK1/2 (C-19): sc-290. Western blot analysis of NCK1/2 expression in U-937 (A) and A-431 (B) whole cell lysates.

NCK1/2 (G-12) - sc-166425 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCK1/2 (G-12): sc-166425. Western blot analysis of NCK1/2 expression in U-937 whole cell lysate.

Anti-Nck 1/2 antibody - ab77165 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nck 1/2 antibody (ab77165) at 1/2000 dilutionLane 1 : U937 cell lysateLane 2 : A431 cell lysateLane 3 : NIH3T3 cell lysateLane 4 : C6 cell lysate

Anti-Nck 1/2 antibody [AF6D7] - ab80620 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone AF6D7
All lanes : Anti-Nck 1/2 antibody [AF6D7] (ab80620) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : U937 cell lysateLane 3 : A431 cell lysateLane 4 : SK-N-MC cell lysateLane 5 : Ramos cell lysateLane 6 : PC12 cell lysate

Anti-Nck antibody - ab168940 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-Nck antibody (ab168940) at 1 µg/ml + A431 cell lysate at 50 µgdeveloped using the ECL technique

Anti-Nck antibody - ab14589 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Nck antibody - ab14587 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Nck antibody - ab72024 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nck antibody (ab72024) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysate

Anti-Nck antibody - ab134319 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labelling Nck with ab134319 at 1/200 dilution, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Nck antibody - ab116544 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Nck antibody (ab116544) at 1/100 dilution + K562 cell line lysates at 35 µg

Anti-Nck antibody - ab55499 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Nck antibody [11D10] - ab77124 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 11D10
All lanes : Anti-Nck antibody [11D10] (ab77124) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : RD cell lysateLane 3 : PC12 cell lysateLane 4 : NIH3T3 cell lysate

Anti-Nck antibody [5B7] - ab129367 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5B7
ab129367 staining Nck antigen by ELISA.

Anti-Nck antibody [NC-20 (same as 20B-1H9)] - ab44950 from Abcam

Prices $370.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone NC-20 (same as 20B-1H9)

Anti-Nck antibody [Y531] - ab32120 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y531
Overlay histogram showing Jurkat cells stained with ab32120 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32120, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NCK1 / NCK Antibody (aa62-92, Biotin) - LS-C223410 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCK1 / NCK Antibody (aa62-92, FITC) - LS-C223413 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCK1 / NCK Antibody (aa52-101) - LS-C151733 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NCK1 / NCK antibody Western Blot of NCI-H226.
Search more