Anti-NCKIPSD antibody (ab88467) at 1 µg/ml + rat brain tissue lysate at 50 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Anti-NCKIPSD antibody (ab88467) at 1 µg/ml + HeLa cell lysate at 50 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
All lanes : Anti-NCKIPSD antibody (ab88467) at 1 µg/mlLane 1 : NCKIPSD transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 50 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution