All lanes : Anti-NCR3 antibody [EPR14509] (ab186425) at 1/1000 dilutionLane 1 : THP1 cell lysateLane 2 : NCI-H460 lysateLane 3 : Rat brain lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-NCR3 antibody [EPR14509] (ab186425) at 1/1000 dilution + K562 lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of NCR3 in K562 cell lysate immunoprecipitated using ab186425 at 1/50 dilution (Lane 1). Lane 2: PBS instead of K562 lysate. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500 dilution.