Anti-NCS1 antibody [EPR7699]

• Supplier: Abcam
• Catalog: ab129166
• Prices: $378.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7699
• Applications: WB IP IHC-P FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to residues on the C terminal of Human NCS1
• Description: Rabbit Monoclonal
• Gene: NCS1
• Conjugate: Unconjugated

Product images

Overlay histogram showing SH-SY5Y cells stained with ab129166 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129166, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-NCS1 antibody [EPR7699] (ab129166) at 1/1000 dilutionLane 1 : U-87 MG cell lysatesLane 2 : SH-SY5Y cell lysatesLane 3 : A549 cell lysatesLane 4 : 293T cell lysatesLane 5 : Fetal brain tissue lysatesLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

ab129165, at 1/250 dilution staining NCS1 in paraffin-embedded Human brain tissue, by Immunohistochemistry.