NCS1

Gene Symbol NCS1
Entrez Gene 23413
Alt Symbol FLUP, FREQ
Species Human
Gene Type protein-coding
Description neuronal calcium sensor 1
Other Description frequenin homolog|frequenin-like protein|frequenin-like ubiquitous protein
Swissprots Q9UK26 E9PAY3 P62166 P36610
Accessions EAW87926 EAW87927 P62166 AF134479 AAP97256 AF186409 AAF01804 AI521726 AI564128 AI768193 AK023889 AK024709 AL161981 BC004856 AAH04856 BC008698 BM011362 BQ880305 BU150740 CF453730 CK301145 DA092531 X84048 CAA58867 NM_001128826 NP_001122298 NM_014286 NP_055101
Function Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel (By similarity). {ECO:0000250}.
Subcellular Location Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein. Note=Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.

NCS1 (D12D2) XP ® Rabbit mAb - 8237 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D12D2
Western blot analysis of extracts from mouse brain, rat brain, and human cerebellum using NCS1 (D12D2) XP ® Rabbit mAb.

NCS-1 (G-4) - sc-376206 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NCS-1 (G-4): sc-376206. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

NCS-1 (C-15) - sc-13167 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NCS-1 (C-15): sc-13167. Western blot analysis of human recombinant NCS-1 fusion protein.

NCS-1 (L-19) - sc-13166 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NCS-1 (FL-190) - sc-13037 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NCS-1 (FL-190): sc-13037. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.

NCS-1 (D-18) - sc-13165 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NCS-1 (1) - sc-136000 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
NCS-1 (1): sc-136000. Western blot analysis of NCS-1 expression in rat cerebrum tissue extract.

Anti-Frequenin antibody - ab97754 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Frequenin antibody (ab97754) at 1/200 dilutionLane 1 : A431 whole cell lysateLane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-NCS1 antibody - ab101814 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-NCS1 antibody - ab168632 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-NCS1 antibody (ab168632) at 1/500 dilutionLane 1 : NCS1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-NCS1 antibody - ab116230 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab116230, at 5 µg/ml, staining NCS1 in formalin fixed, paraffin embedded Human brain (cerebellum) tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Anti-NCS1 antibody - ab135764 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NCS1 antibody (ab135764) at 1/50 dilution + MCF7 cell lysate at 35 µg

Anti-NCS1 antibody [EPR7699] - ab129166 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7699
Overlay histogram showing SH-SY5Y cells stained with ab129166 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129166, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-NCS1 / Neuronal Calcium Sensor Antibody (C-Terminus) - LS-C129161 from LifeSpan Bioscience

Prices $415.00
Sizes 1 ml
Host Sheep
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, FITC) - LS-C257391 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, Biotin) - LS-C257392 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, Azide-free, HRP) - LS-C257394 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, PE) - LS-C257395 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (N-Terminus) - LS-C30071 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NCS1 / NCS-1 antibody LS-C30071 was used in IHC to stain formalin-fixed, paraffin-embedded human brain.

Anti-NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, AP) - LS-C259428 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (aa1-30, APC) - LS-C259430 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (Internal, AP) - LS-C277407 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (Internal, APC) - LS-C277408 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (Internal, Biotin) - LS-C277409 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NCS1 / Neuronal Calcium Sensor Antibody (Internal, FITC) - LS-C277410 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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