All lanes : Anti-NCX1 antibody (ab135735) at 1/100 dilutionLane 1 : HL60 cell line lysateLane 2 : K562 cell line lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labelling NCX1 with ab135735 at 1/50 dilution, followed by peroxidase conjugated secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells labelling NCX1 with ab135735 at 1/10 dilution (green) compared to negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.