Western blot analysis of total cell lysates from SK-N-MC cells, treated with forskolin (FSK) for 30 minutes, or the lysate was treated with λ phosphatase for 1 hour, using Phospho-β-Catenin (Ser552) Antibody (upper) or β-Catenin Antibody (Amino-terminal Antigen) #9581 (bottom).
COS-7 cells were transfected with cDNAs for the DYKDDDDK-tagged wild type (WT) β-catenin or for Ser-to-Ala β-catenin mutants as indicated. Cells were stimulated with 10 mM forskolin (FSK) for 5 minutes and lysed. β-catenin or its mutants were immunoprecipitated with DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG ® M2 Antibody) #2368 and analyzed by western blotting with Phospho-β-Catenin (Ser552) Antibody #9566, Phospho-β-Catenin (Ser675) Antibody #9567, or DYKDDDDK Tag Antibody as indicated (Figures provided by Drs. Sebastien Taurin and Nickolai Dulin, Department of Medicine / Pulmonary, The University of Chicago).