Anti-Nectin 2 antibody [B-C12]
| Name | Anti-Nectin 2 antibody [B-C12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab47171 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | B-C12 |
| Applications | IHC-F ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | KG-1 cell line |
| Description | Mouse Monoclonal |
| Gene | PVRL2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
A typical staining pattern with ab47171 monoclonal antibody of KG-1 cell line
ICC/IF image of ab47171 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47171, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.