Anti-Nectin 2 antibody [EPR6717]

• Supplier: Abcam
• Catalog: ab135246
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR6717
• Applications: WB IP FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide, corresponding to residues in the intracellular domain of Human Nectin 2
• Description: Rabbit Monoclonal
• Gene: PVRL2
• Conjugate: Unconjugated

Product images

Overlay histogram showing HepG2 cells stained with ab135246 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab135246, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Nectin 2 antibody [EPR6717] (ab135246) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : 293T cell lysateLane 3 : ECV 304 cell lysateLane 4 : SW480 cell lysateLysates/proteins at 10 µg per lane.

Immunofluorescent sanalysis of SW480 cells labelling Nectin 2 with ab135246 at 1/250 dilution.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD