Anti-NEDD1 antibody
| Name | Anti-NEDD1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57336 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Xenopus |
| Antigen | Recombinant fragment: AGVASSLSEK IADSIGNNRQ NAPLTSIQIR FIQNMIQETL DDFREACHRD IVNLQVEMIK QFHMQLNEMH SLLERYSVNE GLVAEIERLR EENKRLRAHF , corresponding to amino acids 561-661 of Human NEDD1 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | NEDD1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab57336 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57336, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/28774_ab57336-4-ab57336FC.jpg)
Overlay histogram showing HeLa cells stained with ab57336 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57336, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
NEDD1 antibody (ab57336) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human placenta.
Anti-NEDD1 antibody (ab57336) staining NEDD1 in immunocytochemistry/immunofluorescence in Xenopus laevis cytoplasmic egg extracts. The localization by IF is what would be expected, at spindle pole and microtubules.See Abreview
Product References
Importance of the CEP215-pericentrin interaction for centrosome maturation during - Importance of the CEP215-pericentrin interaction for centrosome maturation during
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Interaction proteomics identify NEURL4 and the HECT E3 ligase HERC2 as novel - Interaction proteomics identify NEURL4 and the HECT E3 ligase HERC2 as novel
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CEP192 interacts physically and functionally with the K63-deubiquitinase CYLD to - CEP192 interacts physically and functionally with the K63-deubiquitinase CYLD to
Gomez-Ferreria MA, Bashkurov M, Mullin M, Gingras AC, Pelletier L. Cell Cycle. 2012 Oct 1;11(19):3555-8.
Apaf1 plays a pro-survival role by regulating centrosome morphology and function. - Apaf1 plays a pro-survival role by regulating centrosome morphology and function.
Ferraro E, Pesaresi MG, De Zio D, Cencioni MT, Gortat A, Cozzolino M, Berghella L, Salvatore AM, Oettinghaus B, Scorrano L, Perez-Paya E, Cecconi F. J Cell Sci. 2011 Oct 15;124(Pt 20):3450-63.
PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset - PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset
Lee K, Rhee K. J Cell Biol. 2011 Dec 26;195(7):1093-101.
Sam68 regulates translation of target mRNAs in male germ cells, necessary for - Sam68 regulates translation of target mRNAs in male germ cells, necessary for
Paronetto MP, Messina V, Bianchi E, Barchi M, Vogel G, Moretti C, Palombi F, Stefanini M, Geremia R, Richard S, Sette C. J Cell Biol. 2009 Apr 20;185(2):235-49.
The alternative TrkAIII splice variant targets the centrosome and promotes - The alternative TrkAIII splice variant targets the centrosome and promotes
Farina AR, Tacconelli A, Cappabianca L, Cea G, Panella S, Chioda A, Romanelli A, Pedone C, Gulino A, Mackay AR. Mol Cell Biol. 2009 Sep;29(17):4812-30.