NEDD1

Gene Symbol NEDD1
Entrez Gene 121441
Alt Symbol GCP-WD, TUBGCP7
Species Human
Gene Type protein-coding
Description neural precursor cell expressed, developmentally down-regulated 1
Other Description NEDD-1|neural precursor cell expressed developmentally down-regulated protein 1|protein NEDD1
Swissprots G3V3F1 Q8NA30 B4E145 Q8NHV4 B0AZN0
Accessions EAW97577 EAW97578 EAW97579 EAW97580 Q8NHV4 AA236927 AK093221 BAC04099 AK290058 BAF82747 AK303656 BAG64657 AK315821 BAF98712 AL832466 AL832468 BC020799 AAH20799 BC026168 BC027605 AAH27605 DB074571 DQ891606 ABM82532 DQ894799 ABM85725 XM_005268644 XP_005268701 XM_006719237 XP_006719300 XM_011537903 XP_011536205 XM_011537904 XP_011536206 XM_011537905 XP_011536207 XM_011537906 XP_011536208 XM_011537907 XP_011536209 NM_001135175 NP_001128647 NM_001135176 NP_001128648 NM_001135177 NP_001128649 NM_152905 NP_690869
Function Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. {ECO:0000269|PubMed:19029337, ECO:0000269|PubMed:19509060}.
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:19029337}.

NEDD1 (H-300) - sc-67263 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

NEDD1 (H-3) - sc-398733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NEDD1 (H-3): sc-398733. Western blot analysis of NEDD1 expression in HeLa (A) and Jurkat (B) whole cell lysates.

NEDD1 (Y-16) - sc-54127 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NEDD1 (S-18) - sc-54126 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NEDD1 (39-J) - sc-100961 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
NEDD1 (39-J): sc-100961. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human placenta tissue showing membrane localization.

Anti-NEDD1 antibody - ab57336 from Abcam

Prices $380.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab57336 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57336, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NEDD1 antibody - ab56317 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-NEDD1 antibody (ab56317) at 0.1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg

Anti-NEDD1 antibody - ab181654 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD1 Antibody (aa561-661, Azide-free) - LS-C328008 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-NEDD1 Antibody - LS-C153764 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-NEDD1 in Western blot of Immunochemicals' Anti-NEDD1 Antibody shows detection of a 73 kDa band corresponding to endogenous NEDD1 in lysates of S phase HeLa cells silenced for either control Luciferase or NEDD1. In right lane (NEDD1i): lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-NEDD1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

Anti-NEDD1 Antibody (N-Terminus) - LS-C111478 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NEDD1 antibody LS-C111478 Western blot of Fetal Spleen lysate.

Anti-NEDD1 Antibody (aa527-540) - LS-C55153 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.1 ug/ml) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-NEDD1 Antibody (clone 7D10) - LS-C133833 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7D10
Immunoperoxidase of monoclonal antibody to NEDD1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.75 ug/ml]

NEDD1 Antibody (7D10) - H00121441-M05 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7D10
Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in NIH/3T3 ( Cat # L018V1 ).

NEDD1 Antibody - NBP1-55320 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NEDD1 Antibody [NBP1-55320] - Titration: 0.2-1 ug/ml, Positive Control: Human Spleen.

NEDD1 Antibody - NB100-55420 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

NEDD1 Antibody - NBP1-83377 from Novus Biologicals

Prices $129.00, $419.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NEDD1 Antibody [NBP1-83377] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line PC-3

NEDD1 Polyclonal Antibody - bs-7813R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD1 antibody produced in goat - SAB2500678 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-NEDD1: <B>Cat. No. SAB2500678</B>: (0.1 μg/mL) staining of HeLa cell lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-NEDD1 antibody produced in rabbit - HPA038591 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NEDD1: <B>Cat. No. HPA038591</B>: Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Anti-NEDD1, (N-terminal) antibody produced in rabbit - SAB2103622 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-NEDD1, antibody produced in rabbit: <B>Cat. No. SAB2103622</B>: Immunoblotting analysis of NEDD1 in fetal spleen tissue lysate with NEDD1 anitbody at 1 μg/mL.

Monoclonal Anti-NEDD1 antibody produced in mouse - WH0121441M5 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7D10
<B>Immunoblotting</B><BR/>Monoclonal Anti-NEDD1, <B>Cat. No. WH0121441M5</B> used at the antibody concentration: 1 ~ 5 μg/mL. Specific band of ~71.9 kDa using Hela NE lysate.

Monoclonal Anti-NEDD1, (C-terminal) antibody produced in mouse - SAB1405351 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3H7
<B>Immunoblotting</B><BR/>Monoclonal Anti-NEDD1: <B>Cat. No. SAB1405351</B>: Antibody concentration: 1-5 μg/mL. Specific band of ~72 kDa using HeLa NE lysate.

NEDD1 antibody - N-terminal region (ARP53120_P050) - ARP53120_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NEDD1-Antibody-monoclonal-M05 - AT3009a from Abgent, a WuXi AppTec company

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7D10
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
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