All lanes : Anti-NEDD4 antibody [EPR14737] - C-terminal (ab188867) at 1/50000 dilutionLane 1 : MCF-7 cell lysateLane 2 : A549 cell lysateLane 3 : HeLa cell lysateLane 4 : Raw264.7 cell lysateLane 5 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling NEDD4 with ab188867 at 1/40 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot analysis of NEDD4 in HeLa cell lysate immunoprecipitated using ab188867 at 1/50 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.