NEDD4

Gene Symbol NEDD4
Entrez Gene 4734
Alt Symbol NEDD4-1, RPF1
Species Human
Gene Type protein-coding
Description neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
Other Description E3 ubiquitin-protein ligase NEDD4|cell proliferation-inducing gene 53 protein|neural precursor cell expressed developmentally down-regulated protein 4|receptor-potentiating factor 1
Swissprots P46934 D6RF89 B7ZM59 B4E2R7 B7ZM60 B9EGN5 A1KY35 A6ND72 A7MD29
Accessions EAW77495 EAW77496 EAW77497 P46934 AK304394 BAG65229 AK307541 AL832063 AL832359 AL833293 AL833300 AY550969 AAT52215 BC035707 BC047035 BC136605 AAI36606 BC144284 AAI44285 BC144285 AAI44286 BC152452 AAI52453 BC152562 AAI52563 D42055 BAA07655 DB451823 EU832821 ACE87832 GQ129222 ACT64356 XM_011521624 XP_011519926 XM_011521625 XP_011519927 XM_011521626 XP_011519928 XM_011521627 XP_011519929 NM_001284338 NP_001271267 NM_001284339 NP_001271268 NM_001284340 NP_001271269 NM_006154 NP_006145 NM_198400 NP_940682 NR_104302
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the degradation of VEGFR- 2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of E
Subcellular Location Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). May be recruited to exosomes by NDFIP1. {ECO:0000250, ECO:0000269|PubMed:18819914}.
Top Pathways Ubiquitin mediated proteolysis, Endocytosis, Epstein-Barr virus infection

NEDD4 (D82F4) Rabbit mAb - 5344 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D82F4
Western blot analysis of extracts from MCF7 and H-4-II-E cells using NEDD4 (D82F4) Rabbit mAb.

NEDD4 Antibody - 2740 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysates from NIH/3T3, C6, H-4-II-E, U87, A549 and COS cells, using NEDD4 antibody.

NEDD4 (C5F5) Rabbit mAb - 3607 from Cell Signaling Technology

Prices $256.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C5F5
Western blot analysis of extracts from various cell lines using NEDD4 (C5F5) Rabbit mAb.

NEDD4 (D-17) - sc-14428 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NEDD4-1 (H-135) - sc-25508 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NEDD4-1 (H-135): sc-25508. Western blot analysis of NEDD4 expression in KNRK whole cell lysate.

NEDD4-1 (A-16) - sc-14429 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NEDD4 (A-16): sc-14429. Immunofluorescence staining of methanol-fixed Caki-1 cells showing cytoplasmic localization.

Anti-NEDD4 antibody - ab14592 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4 antibody - ab27979 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NEDD4 antibody (ab27979) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-NEDD4 antibody - ab76341 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-NEDD4 antibody - ab76336 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-NEDD4 antibody - ab117826 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of Human NEDD4 in HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab117826 at 6 µg/mg of lysate. Subsequent WB detection was done using another anti Human NEDD4 antibody. Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-NEDD4 antibody [EPR14737] - C-terminal - ab188867 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR14737
All lanes : Anti-NEDD4 antibody [EPR14737] - C-terminal (ab188867) at 1/50000 dilutionLane 1 : MCF-7 cell lysateLane 2 : A549 cell lysateLane 3 : HeLa cell lysateLane 4 : Raw264.7 cell lysateLane 5 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-NEDD4 Antibody (aa211-349) - LS-C129499 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-NEDD4 Antibody (Internal) - LS-C60145 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Nedd4 Antibody - Western Blot. Western blot of affinity purified anti-Nedd4 antibody shows detection of a 115 kD band corresponding to endogenous Nedd4 (arrowhead) in MDA-MB-435S cell lysates. The blot was blocked with B501-0500 5% BLOTTO overnight at 4C. Primary antibody was used at a 1:350 dilution in 5% BLOTTO followed by reaction with a 1:20000 dilution of HRP goat anti-rabbit IgG in #MB-070 Blocking Buffer for Fluorescent Western Blot. ECL was used for detection.

Anti-NEDD4 Antibody (aa950-1000) - LS-C148765 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148765 at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution were used for this test.

Anti-NEDD4 Antibody (aa725-775) - LS-C289949 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4 Antibody (aa395-462) - LS-C188043 from LifeSpan Bioscience

Prices $615.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4 Antibody (aa825-875) - LS-C289948 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4 Antibody (aa324-373) - LS-C110750 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NEDD4 antibody LS-C110750 Western blot of MCF7 cell lysate.

Anti-NEDD4 Antibody (aa200-250) - LS-C148723 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using LS-C148723 at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution were used for this test.

Anti-NEDD4 Antibody - LS-C330999 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of C6 cells tissue, using NEDD4 antibody.

Anti-NEDD4 Antibody (aa1288-1319) - LS-C164818 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
NEDD4 Antibody IHC of formalin-fixed and paraffin-embedded brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-NEDD4 Antibody (aa395-462) IHC-plus™ - LS-B2327 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-NEDD4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2327 dilution 1:100.

Anti-NEDD4 Antibody (aa634-878) - LS-C178609 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

NEDD4 Antibody - NBP2-27201 from Novus Biologicals

Prices $129.00, $279.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: NEDD4 Antibody [NBP2-27201] - Analysis of NEDD4 in mouse embryo brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27201. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
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