All lanes : Anti-NEIL2 antibody [EPR13944] (ab180576) at 1/50000 dilutionLane 1 : THP1 whole cell lysateLane 2 : HepG2 whole cell lysateLane 3 : Jurkat whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-NEIL2 antibody [EPR13944] (ab180576) at 1/10000 dilution + Human skeletal muscle lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling NEIL2 with ab180576 at 1/10 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab180576 at 1/40 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.