Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95176 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-NEK4 antibody (ab95176) at 0.04 µg/mlLane 1 : HeLa cell lysate at 50 µg/mlLane 2 : HeLa cell lysate at 15 µg/mlLane 3 : HeLa cell lysate at 5 µg/ml
Immunoprecipitation of NEK4 from HeLa cell lysate (1mg) using ab95176 at 3µg/mg lysate , followed by Western blotting of 20% of the immunoprecipitate: (1) ab95176 at 1 µg/ml, (2) control IgGDetection: Chemiluminescence with exposure times of 30 seconds.