All lanes : Anti-NELFe antibody [EPR11600] (ab170104) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : T47-D cell lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Immunofluorescence analysis of HepG2 cells labeling NELFe with ab170104 at a 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or 1X PBS (negative control) (lane 2) using ab170104, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.