NELFE

Gene Symbol	NELFE
Entrez Gene	7936
Alt Symbol	D6S45, NELF-E, RD, RDBP, RDP
Species	Human
Gene Type	protein-coding
Description	negative elongation factor complex member E
Other Description	RD RNA binding protein\|RD RNA-binding protein\|RNA-binding protein RD\|major histocompatibility complex gene RD\|negative elongation factor E\|negative elongation factor polypeptide E\|nuclear protein
Swissprots	Q96F56 Q5JP74 P18615 B4DUN1 A2BE08 B4DYX9 Q5JP75 Q9NPK2
Accessions	AAA36308 AAB67979 AAK29466 CAB89308 EAX03551 EAX03552 EAX03553 P18615 AK300717 BAG62393 AK300898 BAH13370 AK302652 BAG63891 AK303152 BAG64255 BC025235 AAH25235 BC050617 AAH50617 BU935034 CD579634 DQ896847 ABM87846 JQ313904 AFH41798 L03411 AAC37523 X16105 CAA34231 XM_006715205 XP_006715268 XM_006725521 XP_006725584 XM_006725739 XP_006725802 XM_006725936 XP_006725999 XM_006726037 XP_006726100 XM_006726125 XP_006726188 XM_011514913 XP_011513215 XM_011547314 XP_011545616 XM_011547716 XP_011546018 XM_011548102 XP_011546404 XM_011548301 XP_011546603 XM_011548486 XP_011546788 NM_002904 NP_002895
Function	Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. {ECO:0000269\|PubMed:10199401, ECO:0000269\|PubMed:11940650, ECO:0000269\|PubMed:12612062}.
Subcellular Location	Nucleus.
Tissue Specificity	Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas. {ECO:0000269\|PubMed:12612062}.

NELF-E (F-9) - sc-377052 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

NELF-E (F-9): sc-377052. Western blot analysis of NELF-E expression in Jurkat (A) and HeLa (B) nuclear extracts.

NELF-E (H-140) - sc-32912 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

NELF-E (H-140): sc-32912. Western blot analysis of NELF-E expression in 293T whole cell lysate.

NELF-E (K-20) - sc-19560 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-NELFe antibody - ab28038 from Abcam

Prices	$378.00
Sizes	200 µl
Host	Sheep
Clonality	Polyclonal

All lanes : Anti-NELFe antibody (ab28038) at 1/1000 dilutionLane 1 : NELFe-GST fusion proteinLane 2 : HeLa cell nuclear extractSecondarydonkey anti-sheep peroxidase linked at 1/20000 dilution

Anti-NELFe antibody - ab84986 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ab84986 at 1/250 dilution staining NELFe in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

Anti-NELFe antibody [EPR11600] - ab170104 from Abcam

Prices	$385.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR11600

All lanes : Anti-NELFe antibody [EPR11600] (ab170104) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : T47-D cell lysateLane 3 : HepG2 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-NELFE / RD / RDBP Antibody (aa75-125) - LS-C288681 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human NELFE by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NELFE antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NELFE was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Anti-NELFE / RD / RDBP Antibody (aa35-84) - LS-C110517 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

RDBP antibody LS-C110517 Western blot of Fetal Spleen lysate.

Anti-NELFE / RD / RDBP Antibody (N-Terminus) - LS-C110518 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

RDBP antibody LS-C110518 Western blot of Fetal Brain lysate.

Anti-NELFE / RD / RDBP Antibody - LS-C346175 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence blot of U20S cell using NELFE antibody.

Anti-NELFE / RD / RDBP Antibody - LS-C335441 from LifeSpan Bioscience

Prices	$305.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of various cell lines, using NELFE antibody.

Anti-NELFE / RD / RDBP Antibody (aa330-380) - LS-C286617 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human NELFE by Immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-NELFE used at a dilution of 1:250.

Anti-NELFE / RD / RDBP Antibody (aa330-380) - LS-C288683 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human NELFE by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NELFE used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

NELF-E Antibody - NBP1-88036 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: NELF-E Antibody [NBP1-88036] - Analysis in human cell line MOLT-4.

NELF-E Antibody - NBP1-57196 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: NELF-E Antibody [NBP1-57196] - Human Brain lysate, concentration 0.2-1 ug/ml.

NELF-E Antibody - NBP1-57127 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

NELF-E Antibody - NBP1-05978 from Novus Biologicals

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: NELF-E Antibody [NBP1-05978] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NELFE antibody used for IP at 3 mcg/mg lysate. NELFE was also immunoprecipitated by rabbit anti- NELFE antibody NBP1-05979, which recognizes a downstream epitope.

Anti-NELFE antibody produced in rabbit - HPA007187 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-AL844853.23: <B>Cat. No. HPA007187</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human cerebral cortex shows strong nuclear positivity in neuronal cells.

Anti-RDBP, (N-terminal) antibody produced in rabbit - SAB2103787 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-RDBP, antibody produced in rabbit: <B>Cat. No. SAB2103787</B>: Immunoblotting analysis of RDBP in fetal spleen tissue lysate with RDBP anitbody at 1 μg/mL.

Anti-RDBP antibody produced in rabbit - SAB2101972 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-RDBP: <B>Cat. No.SAB2101972</B>: RDBP in Fetal Brain tissue lysate with RDBP antibody at 1.0 μg/mL.

RDBP antibody - N-terminal region (ARP40430_P050) - ARP40430_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

RDBP antibody - N-terminal region (ARP40431_P050) - ARP40431_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

RDBP antibody - 70R-19836 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

NELFE Polyclonal Antibody - A4192 from ABclonal

Prices	$200.00, $480.00
Sizes	50 µg, 200 µg
Host	Rabbit
Clonality	Polyclonal

NELFE Polyclonal Antibody - A7006 from ABclonal

Prices	$130.00, $220.00, $380.00
Sizes	50 µl, 100 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

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