Anti-Nestin antibody [3k1]

• Supplier: Abcam
• Catalog: ab6320
• Prices: $401.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 3k1
• Applications: IHC-F IHC IHC-F ICC/IF FC ELISA WB IP ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Nestin-transfected NS0 cells transfected with a human Nestin fragment aa residues 618-1618
• Description: Mouse Monoclonal
• Gene: NES
• Conjugate: Unconjugated

Product images

Tumorigenicity of human glioma-derived cells. Representative immunohistochemical analysis of brain tumors generated after orthotropic injection of isolated human glioblastoma cells. Immunofluorescence of mouse brains cryosections labeled with anti-human nestin antibody. A, diffuse infiltration of the injected and contralateral hemisphere (GBM 1). B, cells infiltrating the striatum ipsilateral and contralateral to the side of injection (GBM 2). Scale bar, 100 µm. GBM xenografts recapitulate the morphology of corresponding tumors in human cancer patients. For xenograft tumor analysis, mice were sacrificed, and cryopreserved brain sections were cut using a 10-µm cryostat. Staining with hematoxylin-eosin identified sections bearing tumors. Briefly, brain sections were mounted on slides and stained with hematoxylin for 40 seconds and then counterstained with alcoholic eosin for 30 seconds. Cryosections containing tumors were permeabilized in PBS containing 0.5% Triton X-100 and

ab6320 staining Nestin in Human glioblastoma in mouse brain by Immunohistochemistry - Wholemount. Samples were incubated with primary antibody (1/1000 in diluent) for 16 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

Product References

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