NES

Gene Symbol NES
Entrez Gene 10763
Alt Symbol Nbla00170
Species Human
Gene Type protein-coding
Description nestin
Other Description -
Swissprots Q3LIF5 P48681 Q5SYZ6 O00552
Accessions AAB64426 CAA46780 EAW52923 EAW52924 EAW52925 EAW52926 P48681 AB073350 BAE45713 AF086454 AK025494 BAB15153 AL120767 BC032580 AAH32580 BC051373 BC108285 AAI08286 BC142611 AAI42612 BF340882 BG912782 BQ678387 BU554136 GU014842 ACX32329 NM_006617 NP_006608
Function Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). {ECO:0000250}.
Tissue Specificity CNS stem cells.

nestin (10c2) - sc-23927 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
nestin (10c2): sc-23927. Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.

nestin (D-9) - sc-377380 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
nestin (D-9): sc-377380. Western blot analysis of nestin expression in IMR-32 whole cell lysate.

p-nestin (A-4) - sc-377538 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of nestin phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) SH-SY5Y whole cell lysates. Antibodies tested include p-nestin (A-4): sc-377538 (A,B) and nestin (C-20): sc-21247 (C,D).

nestin (C-20) - sc-21247 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
nestin (C-20): sc-21247. Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.

nestin (G-20) - sc-21248 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
nestin (G-20): sc-21248. Western blot analysis of nestin expression in SK-N-SH (A) and SH-SY5Y (B) whole cell lysates.

nestin (2C1.3A11) - sc-73489 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

nestin (H-85) - sc-20978 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
nestin (H-85): sc-20978. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.

nestin (5C93) - sc-71665 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-nestin (Thr 1583) - sc-33880 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of nestin phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) mouse embryonic brain tissue extract. Antibodies tested include p-nestin (Thr 1495): sc-33880 (A,B) and nestin (C-20): sc-21247 (C,D).

p-nestin (Thr 316) - sc-33879 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-Nestin antibody - ab93666 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab93666, at a 1/200 dilution, staining Nestin in formalin fixed, paraffin embedded Human liver carcinoma tissue by Immunohistochemistry.

Anti-Nestin antibody - ab134017 from Abcam

Prices $378.00
Sizes 150 µg
Host Chicken
Clonality Polyclonal
Immunohistochemical analysis of a tissue section through an e13 mouse brain labelling Nestin (green staining) in neural stem cells of the ventricular zone of the CNS with ab134017 at 1/10000 dilution. DAPI (blue staining) allows visualization of nuclei.

Anti-Nestin antibody - ab82375 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Nestin antibody (ab82375) at 1/50 dilution + HepG2 cell line lysates at 35 µg

Anti-Nestin antibody - ab85684 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Nestin antibody (ab85684) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-Nestin antibody [10C2] - ab22035 from Abcam

Prices $394.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 10C2
ab22035 staining human brain tissue cells by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 21°C.  The primary antibody was diluted 1/100 and incubated with the samples for 1 hour at 21°C.  A FITC conjugated goat anti-mouse IgG antibody, diluted 1/250, was used as the secondary.See Abreview

Anti-Nestin antibody [2C1.3A11] - ab18102 from Abcam

Prices $388.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C1.3A11
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of migrating tumor cells labelling Nestin with ab18102 at 2µg/ml. Cytoplasm and membrane positive staining shown.

Anti-Nestin antibody [2C1.3A11] (FITC) - ab187846 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 2C1.3A11

Anti-Nestin antibody [2C1.3A11] (HRP) - ab187845 from Abcam

Prices $372.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 2C1.3A11

Anti-Nestin antibody [3k1] - ab6320 from Abcam

Prices $401.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3k1
Tumorigenicity of human glioma-derived cells. Representative immunohistochemical analysis of brain tumors generated after orthotropic injection of isolated human glioblastoma cells. Immunofluorescence of mouse brains cryosections labeled with anti-human nestin antibody. A, diffuse infiltration of the injected and contralateral hemisphere (GBM 1). B, cells infiltrating the striatum ipsilateral and contralateral to the side of injection (GBM 2). Scale bar, 100 µm. GBM xenografts recapitulate the morphology of corresponding tumors in human cancer patients. For xenograft tumor analysis, mice were sacrificed, and cryopreserved brain sections were cut using a 10-µm cryostat. Staining with hematoxylin-eosin identified sections bearing tumors. Briefly, brain sections were mounted on slides and stained with hematoxylin for 40 seconds and then counterstained with alcoholic eosin for 30 seconds. Cryosections containing tumors were permeabilized in PBS containing 0.5% Triton X-100 and

Anti-Nestin antibody [EPR1301(2)] - ab176571 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1301(2)
ICC/IF image of ab176571 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab176571 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Nestin antibody [GT623] - ab184543 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone GT623
Immunofluorescent analysis of SKNSH cells (fixed in 4% paraformaldehyde at room temperature for 5 min) labeling Nestin with ab184543 at 1/500 dilution (Green). Blue: Hoechst 33342 staining.

Anti-Nestin antibody [SP103] - ab105389 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP103
Anti-Nestin antibody [SP103] (ab105389) at 1/100 dilution + HeLa cell lysate

Anti-Nestin antibody - Aminoterminal end - ab76684 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab76684, at a 1/50 dilution, staining Nestin in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.

Anti-Nestin antibody - Azide free - ab27952 from Abcam

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab27952 staining rat astrocytes by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Saponin prior to blocking in 10% serum for 1 hour at 20°C.  The primary antibody was diluted 1/500 and incubated with the sample for 18 hours at 4°C.  A Cy3® conjugated goat anti-rabbit IgG antibody diluted 1/400 was used as the secondary.See Abreview

Anti-Nestin antibody - Azide free - ab28944 from Abcam

Prices $379.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of Nestin expression in methanol fixed Ntera2 cells stained using 1:200 anti nestin (ab28944, green). The sample was counterstained with Vimentin (red). The micrograph with yellow staining shows overlapping of nestin and vimentin in the cells. A light phase micrograph shows the same cell as a whole.
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